Using a micropipette l00 uL, test samples solution and ketoconazole solution (standard) was shifted into various non-solidified tubes of Sabouraud agar
None of the samples demonstrated growth of fungi; no colonies grew on the Sabouraud agar
We made subcultures on sheep blood, chocolate, and Sabouraud agar
and performed Kinyoun and Ziehl-Neelsen stains, in which we observed partially acidic alcohol-resistant coccobacilli (Appendix Figure, panels B,C).
The purpose of this article is to provide an insight into the share of dermatophytes, yeasts and non-der-matophyte molds as potential pathogens in OM, grown on Sabouraud agar
without cycloheximide, as well as the prevalence of positive and negative results of DME, and grown isolates, according to patient sex and age.
Naturally growing fungal samples from different sources such as bread, rice and maize were collected from various locations around the University of Veterinary and Animal Sciences, Lahore (Pakistan) and subsequently cultured on Sabouraud agar
. After inoculation of samples on the agar, petri dishes were kept at a temperature of 28+-2AoC for 10 days in the incubator to allow the fungal spores grow in the form of colonies.
Enumeration of biofilm cells after a swab of the well was performed by drop plate technique plating cell suspensions of Escherichia coli, Salmonella Typhimurium, and Candida albicans on Eosin Methylene Blue agar (HiMedia, Mumbai, India), Hecktoen Enteric agar (Himedia Mumbai, India), and Sabouraud agar
(Oxoid, Basingstoke, United Kingdom), respectively.
Fusarium solani (4014) was cultured in Sabouraud agar
and preserved in the Laboratorio de Fisiologia e Bioquimica de Microrganismos (LFBM), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil.
Caption: Figure 2: A hyphal form of Sporothrix schenkii from synovial fluid aspirate cultured on Sabouraud agar
. Narrow branching hyphae (red arrows) giving rise to slender conidiophores (white arrows) at right angles.
Plate media (tryptic soy agar [TSA], blood agar, Sabouraud agar
, and MacConkey agar) were inoculated by rolling the swabs over approximately 20% of the medium surface--the "initial inoculum area." Sterile, disposable plastic loops were used to streak in parallel lines from that initial inoculum area into three other quadrants of the medium surface.
The same strains tested in each Petri dish were seeded in the same order in a straight line on a witness sabouraud agar
without EO and were incubated in the same conditions (control Petri dish).
Mycological examination on Sabouraud agar
was still pending.
The media used in this experiment were Sabouraud Agar
, Chitin Agar, and Tryptic Soy Agar to determine the population of fungi, actinomycetes and bacteria, respectively.