were typically visible within 48 hours of inoculation of a
Sabouraud's dextrose agar plate with their respective conidia.
pachydermatis growth on lipid-enriched
Sabouraud's dextrose agar, and 3 (6%) of 50 human samples were positive (Figure 1).
25ul of each sample was cultured on
Sabouraud's dextrose agar, SDA (Merck, Germany), and incubated at ambient temperature for one week aerobically.
For confirmation, the biopsy tissue was inoculated into
Sabouraud's dextrose agar and was kept at 25[degrees]C.
Specimens were cultured in
Sabouraud's dextrose agar and brain-heart infusion agar with blood, gentamicin, and chloramphenicol and incubated at 30 [degrees] C.
neoformans isolates were grown on
Sabouraud's dextrose agar at 37[degrees]C for 48 h, a loopful of cells from the culture was mixed with sterile deionized water and centrifuged.
In the laboratory after the verification of request and macroscopic examination of the specimen, India ink staining, Grams staining, Cryptococcal Ag test (Latex) and culture on
Sabouraud's Dextrose Agar (SDA) was performed on the same day of reception.
Fungal cultural isolation and identification was done using modified
Sabouraud's dextrose agar and Sunflower seed agar plates with Chloramphenicol (0.
Inoculation was carried out on
Sabouraud's dextrose agar (SDA) and incubated at 37[degrees]C for 7 days or more if required.
Total twenty three cases of onychomycosis were purposively enrolled in the study after microbiological confirmation by potassium hydroxide (KOH) smear and culture on
Sabouraud's dextrose agar (SDA).
Swabs were inoculated on slants of
Sabouraud's dextrose agar, SDA (Merck, Germany), and then incubated at ambient temperature for one week.
Specimens from the lateral wall of vagina were subjected to direct wet mount microscopy and fungal culture on
Sabouraud's dextrose agar.
