7-8: Malpas Yesteryear Rally, Hampton, Malpas, Cheshire, SY14
The gardens are located off the A41 near Malpas (SY14
8HN for Satnav).
For AZFa, AZFb and AZFc regions of the Y chromosomes, PCR reactions were performed by using sY14
, sY86, sY127 and sY254 regions primers, respectively.
Here Shocklach Oviatt CofE Primary School, Shocklach, Malpas, SY14
7BN, is put into focus to show its scores in relation to other schools in the area.
This allowed evaluation of the following sites: sY14
(sex-determining region Y, SRY gene) and ZFY (X-linked gene encoding a zinc-finger protein) for internal control regions; sY84 and sY86 for the AZFa region; sY124, sY127, sY129, sY130, and sY134 for the AZFb region; sY147, sY242, sY254, sY255, SPGY1, sY157, and sY158 for the AZFc region (Supplementary table 1).
The DNA was then subjected to polymerase chain reaction (PCR) and assayed using the following tagged sequences: ZFY (control), sy14
(SRY); AZFa specifically, sy84, sy86, sy625, sy114; AZFb specifically, sy117, sy127, sy129, sy134, sy143; AZFc specifically, sy152, sy147, sy254, sy255, sy157; and finally heterochromatin sy160.
Yapilan Y mikrodelesyon testinde SY133, SY14
(SPY) ye internal kontroller haric incelenen fum bolgelerde delesyon saptandi.
The Cock O'Barton, Barton Road, Barton, nr Farndon SY14
7HU (01829 782277 thecockobarton.co.uk) PAST TASTE TESTS TO TRY 5 Y MORFA, RHOSNEIGR Good quality, great comedy nights (ymorfa.co.uk, 01407 810267) CORN MILL, LLANGOLLEN Stunning setting (01978 869555, brunningandprice.co.uk) 56 HIGH STREET, MOLD excellent night out (01352 759225, 56highst.com) BLUE SKY, BANGOR Style and panache (blueskybangor, 01248 355444) THE CASTLE, CONWY mouth-watering menu (castlewales.co.uk, 01492 582800)
Sex determining region of Y (SRY i.e., STS sY14
) was included as internal control.
Meadow Vale Foods Ltd, Wrexham Road, Malpas, Cheshire SY14
Derek & Tina Rayment, By Appointment, Orchard House, Barton, Farndon, Chershire, SY14