STK3

STK3

A gene on chromosome 8q22.2 that encodes a stress-activated, pro-apoptotic kinase which, following caspase cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. STK3 and STK4 are key components of the Hippo signalling pathway, which controls organ size and suppresses tumours by restricting proliferation and promoting apoptosis. STK3 is also a less preferred gene symbol for what is now designated as STK24, see there.
Mentioned in ?
References in periodicals archive ?
Here, we report that VP1 binds to serine/threonine kinase 3 (STK3), a member of the mammalian STE20-like (MST) kinase family.
Additionally, the amino acid residues in FMDV VP1 that mediate the interaction with STK3 were identified.
The cDNA of porcine STK3 was amplified from PK-15 cells and cloned into the pcDNATM3.1/ myc-His(-)A vector (Invitrogen, Carlsbad, CA, USA) to yield the Myc-tagged expression construct (Myc-STK3).
STK3 that was recovered from the library matched porcine STK3 (National Center for Biotechnology Information [NCBI] reference sequence GACC01000309.1).
Knockdown of STK3 Using a Small Interfering RNA (siRNA).
The FMDV Structural Protein VP1 Interacts with the Porcine Host Protein STK3. To date, the multiple functions of FMDV VP1 during viral infection remain unclear.
To confirm the interaction of VP1 and STK3, HEK293T cells were cotransfected with a Myc-STK3 expressing plasmid and a FLAG-VP1 expressing plasmid or an empty FLAG vector.
To further confirm that the FMDV VP1 and STK3 interaction occurs in vivo, PK-15 cells were transfected with a FLAG-VP1 expressing plasmid or an empty FLAG vector.
An IFA was performed subsequently, and the results indicated an interaction between VP1 and STK3 (Figure 1(e)).
In cultured primary cortical neurons, 4-HNE increased the protein levels of phosphop53 and cell cycle-related proteins (cyclin D3, cyclin D1, and CDC25A), caspase-3 activation, PARP cleavage, calpain activation, serine/threonine kinase 3 (Stk3), and sphingosine phosphate lyase 1 (Sgpl1) upregulation.
The instances nug30/p/v and be s stk3 2/4/v are omitted because the preceding matrix partitioning produces a one-dimensional column partitioning, so that no communication is needed for v.
10 differentially expressed proteins (YWHAQ (14-3-3), YWHAH (14-3-3), ITGB2 (ITGB2), ACTB (F-actin), PPP2R2A (PP2A), STK3 (Mst1/2), PPP1CA (PP1), CSNK1E (CK1<$/e), NF2 (Mer), and PPP2R1A (PP2A)) were associated with Hippo signaling pathway (Figure 4).