Ribosomal Protein S6 Kinase-2 (RPS6KB2), Serine/ Threonine Kinase 17b (STK17B), Nuclear factor of activated T-cells (NFATC), Eukaryotic Translation Elongation Factor 1 Gamma (EEF1G), Immediate early response 5 (IER-5), Eukaryotic translation elongation factor 1 (EEF1) gene and Myeloid cell leukemia sequence 1 (MCL1) are also important for modulating and developing effective immune response (14,22,23).
18 gene specific primers (TLR2, NFKB1, IL2, IL16, TNF, IFNG, CXCR3, CCL1, CD81, CD84, RPS6KB2, STK17B, PRKCB1, MCL1, EEF1G, NFATC4, EEF1 and IER5) were used for real time expression profiling (24) (Table 1).
Immune response genes selected for the study (TLR2, NFKB1, IL2, IL16, TNF, IFNG, CXCR3, CCL1, CD81, CD84, RPS6KB2, STK17B, PRKCB1, MCL1, EEF1G, NFATC4, EEF1 and IER5) were amplified from cDNA of PBMCs (Fig.
Ten genes (TLR2, NFKB 1, TNF, IFNG, IL2, CXCR3, PRKCB1, RPS6KB2, STK17B and EEF1) were found significantly upregulated in PBMC's of indigenous Tharparkar cattle as compared to Vrindavani (Table 2).
In the present investigation, 10 genes under investigation (TLR2, NFKB1, TNF, IFNG, IL2, CXCR3, PRKCB1, RPS6KB2, STK17B and EEF1) displayed significantly higher expression in indigenous Tharparkar as compared to Vrindavani cattle.