DOTATOC shows the highest affinity for the SSTR2 receptor with a weaker affinity for SSTR3
[sup.68]Ga-DOTA peptides bind to SSTRs, in particular SSTR3
and SSTR5, both of which are usually overexpressed in neuroendocrine cells .
CC1 pretreatment was performed using the OptiView method for 8-72 min (except for SSTR3 with protease pretreatment for 4 min), followed by incubation with primary antibodies (SSTR1: 1:3000 (Gramsch, Schwabhausen, Germany), SSTR2A: 1: 500 (Dianova, Hamburg, Germany), SSTR3: 1: 250 (Abcam, Cambridge, United Kingdom), SSTR4: 1: 1000 (Gentex, Zeeland, United States), SSTR5: 1:100 (Abcam, Cambridge, United Kingdom), alpha-subunit: 1: 400 (Immunotech, Prague, Czech Republic), PIT-1: 1:200 (8 min) (Cell Signaling Technology, Danvers, United States)) at 37[degrees]C for 32 min if not stated otherwise.
SSTR3. Somatostatin receptor 3 expression was evaluated in 232 pituitary adenoma samples.
Additionally, SSTR2A and SSTR3 were lower in recurrent PIT-1-positive pituitary adenomas (p = 0.0049 and p = 0.0212, respectively).
While SSTR2 and SSTR4 were not detectable in corticotroph adenomas, one out of three cases showed expression of SSTR1 and one out of two for SSTR3 and SSTR5 [17, 18].
Biological Gene number Partial related methylated genes function Adhesion 580 Sox-2, Shh, Itpkb, Pkd1, Reln, Ptk2b, Nck2, Ass1 Lphn1, Cdh9, Epdr1, Myf5, Pdpn, Has2, Fgfrl1, Fer Secretion 553 Lphn1, Pim3, Fst, Pim3, Pcsk6, Lax1, Sct, Sytl3 Plcd1, Ykt6, Jak1, Stat2, Myc, Csf2, Lifr, Sos1 Proliferation 787 Dixdc1, Lrp6, Edn3, Nkx2, Cyr61, Src, Sox8, Stk4 Ephb1, Sstr3
, Rrm2, Tcf3, Grn, Rhoa, Apc, Nox4, Strn Neuronal 473 Bhlhb9, Cckar, Fzd2, Thy1, Pbx3, regeneration Otx2, Lhx8, Btg2 Klhl1, Dlg2, Pak1, Wnt3, Mif, Tctn1, Evl, Ext1, Als2 Axonal 215 Ifrd1, c-Jun, Bcl2, Tnn, Mbp, Slit3, regeneration Ist1, Drgx, Thy1 Unc5c, Ntrk2, Isl1, Ptk2, Dscam, Atl1, Dnm2, Cxcl12 Figure 3: (a) The distribution of differentially methylated regions (DMRs) peaks in different components of genome.
Among them, GO:0004888 dominantly contains 33 transcripts encoding signal receptors, and these receptors could be further classified into several subgroups: OXTR, LOC431251 and SSTR3
belong to reproductive hormone receptors; CHRM2, ADRA2B, P2RX4, P2RY2, EDNRB2, GABRB2, GABRG2, LOC428961 and NPFFR2 function as receptors mediating neurotransmitters or neuropeptide; GRIN2B and GRIN3A could modulate the efficiency of synaptic transmission; NTRK1 and NTRK2 belong to the receptor tyrosine kinase (RTK) family, and are involved with neurotrophin (GO:0005030--neurotrophin receptor activity; and GO:0043121--neurotrophin binding) (Table 5).
C1QTNF6 (rs229541), which was first identified through a meta-analysis of data from three genome-wide association studies (GWAS) (combined P value = 1.98 x [10.sup.-8]) , is an intronic SNP located on chromosome 22q13 between two genes, C1QTNF6 (C1q and tumor necrosis factor related protein 6) and SSTR3
(somatostatin receptor 3).
Our recent experiments have localised SSTR2, SSTR3 and SSTR5 on the surface of the egg and worm stages of the parasite in host liver and ileum using immunohistochemistry, dot blot and western blotting techniques (Fig.
These chosen sequential epitopes of human and mouse SSTRs (against which the antibodies were raised and screened on parasite sections) were: SSTR2A (RSDSKQDKSRLNETTC), SSTR3 (TAGDKASTLSHL) and SSTR5 (KSGRPQTTLPTRSC).
The sequential epitopes of human and mouse SSTRs (against which the antibodies were raised and used for immunohistochemistry on parasite sections)--SSTR2A (RSDSKQDKSRLNETTC) showed identical stretch of five amino acid residues, SSTR3 (TAGDKASTLSHL) showed identical stretch of ten amino acid residues while sequential epitopes of human and mouse SSTR5 (KSGRPQTTLPTRSC) showed three and four consecutive identical amino acid residues with a non-identical residue in between on comparison with the GPCR family sequence pattern.