The real-time PCR assays were able to detect one copy of the leptin gene or the SRY gene
. Because the DNA concentrations in different individuals differed widely, the relative concentration for each amplicon size was calculated such that the size distribution of DNA in each individual could be compared and summarized.
Amplification products of the SRY gene
were undetectable in control fetuses with the 46,XX karyotype.
Hence, the SRY genes
detected in these serum samples predominantly originated from the nonhematopoietic cells of the male recipients.
In 28 women pregnant with a 46,XY fetus, the mean concentrations of the SRY gene
and hCG were 35.1 copies/mL and 27.8 IU/mL, respectively.
To assess the concentrations of free circulating DNA in plasma of adults as a test of the technique, 38 donors were recruited, and their plasma DNA was analyzed for the presence of the SRY gene
. Cell-free DNA was isolated from 2 mL of plasma from each donor and eluted into a total of 250 [micro]L of buffer.
Primers against SRY gene
and a unique fragment in X chromosomes and ZFX gene were used as internal controls.
In addition, primers for ZFY/ZFX gene and SRY gene
In the search of the SRY gene
during polymerase chain reaction (PCR), the presence of SRY gene
was not found.
No microdeletions were found in AZF regions and SRY gene
Differentiation of the testis is initiated by the SRY gene
located in the short arm of the Y chromosome by Week 7 of gestation.
BMSCs and iPS were isolated from male rats and injected into female rats, enabling detection of the SRY gene
(located on the Y chromosome ([GenBank: FJ168067.1])) as an index of engraftment.