Hepatic insulin receptor deficiency impairs the SREBP-2
response to feeding and statins.
An increase of ER cholesterol above ~5 mol% results in the appearance of PFO-accessible cholesterol and in the disappearance of SREBP-2
in the nucleus controlled by SREBP cleavage-activating protein (SCAP).
Brown, "Akt acutely activates the cholesterogenic transcription factor SREBP-2
," Biochimica et Biophysica Acta (BBA)--Molecular Cell Research, vol.
Thyroid hormone regulation and cholesterol metabolism are connected through Sterol Regulatory Element- Binding Protein-2 (SREBP-2
In cholesterol metabolism, AMPK activation by AICAR inhibited the expression of SREBP-2
and its target genes, HMGCR and 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS), which are key enzymes in cholesterol biosynthesis .
Garcia-Ruiz, "Enhanced free cholesterol, SREBP-2
and StAR expression in human NASH," Journal of Hepatology, vol.
We found that the expression of SREBP-2
, LXR and LDL-R genes all increased with the combined treatment of CA and FA.
Colell, "APP/PS1 mice overexpressing SREBP-2
exhibit combined A[beta] accumulation and tau pathology underlying Alzheimer's disease," Human Molecular Genetics, vol.
As previously discussed, PCSK9 mRNA is under the transcriptional regulation of SREBP-2
; like the LDLR gene, PCSK9 is also regulated by intracellular sterol depletion and inhibitors of HMG CoA R inhibitors (statins)-type medications (53).
Two members of the miR-33 family-miR-33a and miR-33b-which differ in only two nucleotides and which are embedded in the introns of SREBP-2
and SREBP-1, respectively, are present in humans .
From the previous study, the miR96/182/183 cluster is regulated by SREBP-2
which is a function as it activates the expression of many key genes for cholesterol level and uptake.
After that, the membranes were incubated with primary antibodies (SREBP-1 at 1:100 dilution; SREBP-2
at 1:200 dilution; ERa at 1:500 dilution and GAPDH at 1:10,000 dilution) at 4[degrees]C for 16 h.