SREBF1

SREBF1

A gene on chromosome 17p11.2 that encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor, which binds to the sterol regulatory element-1 (SRE1), a decamer flanking the low-density lipoprotein receptor gene and genes involved in sterol biosynthesis. The protein is synthesised as a precursor attached to the nuclear membrane and endoplasmic reticulum; once cleaved, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit precursor cleavage; the mature nuclear form is rapidly catabolised, reducing transcription.
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The relative expression of sterol regulatory element binding transcription factor 1 (SREBF1) in LD muscle in Dorper lambs was not influenced (p>0.05) by dietary supplementation of RO leaves, NS seeds and their combination (Figure 5).
Additionally, a study conducted for the doctoral thesis of Kamineni [9] showed that heat stress significantly upregulated two genes encoding rate-limiting enzymes, lipoprotein lipase (LPL), and sterol regulatory element-binding transcription factor 1 (SREBF1).
We also measured the mRNA levels of the lipogenic transcription factor, sterol regulatory element binding transcription factor 1 (SREBF1), and found that both TPP and IPTP increased its expression during differentiation as compared with MID (Figure 3F).
limiting s-adenosylmethionine and modulating the methylation of SREBF1
Based on [E.sub.r] and [TPM.sub.ma]: [TPM.sub.s] values, 13 genes (ACSS1, ACSS2, ADFP, CD36, FABP3, FASN, GPAM, INSIG1, LPL, SCD5, SPTLC1, SREBF1, and XDH; Table 2; Figure 1) were found to exhibit higher mammary expression over skin or muscle; 6 of these 13 genes (ADFP, FASN, GPAM, LPL, SREBF1, and XDH) further showed preferential expression during adulthood.
In adipose tissue we also measured transcription of Acetyl-CoA Carboxylase Alpha (ACACA), Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1), and Tumor Necrosis Factor alpha (TNFA).
In the 5% [O.sub.2] group, there was a higher abundance of ATF4, CDX2, DDIT3, KEAP1, HSF1, OTX2, PAF1, POU5F1 (OCT4), REST, SREBF1, and XBP1 factors that are related to several cell cycle processes.
Extensive genetic studies have been conducted and identified a group of susceptibility genes for diabetes, such as PTEN [2], SREBF1 [3], JAZF1 [4], BCL2 [5], and FAM19A2 [5].
The TaqMan primer/probe sets for FASN (Hs01005622_ml), acetyl-CoA carboxylase [alpha] (ACACA, Hs01046047_ml), sterol regulatory element binding transcription factor 1 (SREBF1, Hs01088691_ml), stearoyl-CoA desaturase (SCD, Hs01682761_ml), nuclear receptor subfamily 1, group H, member (NR1H) 3 (LXR-[alpha], Hs00172885_ml), NR1H2 (LXR-[beta], Hs01027215_gl), RXR-[alpha] (Hs01067640_ml), and actin, beta (ACTS, Hs01060665_gl) were purchased from Applied Biosystems (CA, USA).
QPCR of SSP1, SREBF1, IL4, ADIPOR1, and RPL4 was performed on Mx3005P (Agilent, Santa Clara, CA, USA) using QuantiFast SYBR Green PCR Master Mix (Qiagen, Hilden, Germany) according to supplier's instruction and analysed in Genex5 Pro as described above.
Gene expression changes favoring the activation of the lipid biosynthesis pathway controlled by sterol regulatory element binding factor 1 (srebf1) would likely lead to the accumulation of fat.