Wild-type and SPAK knockout mice showed significant decreases in blood flow of the tongue, palm, sole, and abdomen at 24 h after LPS administration [Figure 4]a,[Figure 4]b,[Figure 4]c,[Figure 4]d.
Although SPAK [sup]+/+ + LPS group had higher superoxide levels than SPAK [sup]+/+ + vehicle group, it was not significant.
In the SPAK [sup]+/+ + vehicle, SPAK [sup]+/- + vehicle, and SPAK [sup]-/- + vehicle groups, no mortality was observed within 24 h [Table 2].
However, SPAK knockout mice treated with LPS did not show different phenotype regarding all these functional parameters when compared to the wild-type mice treated with LPS.
However, SPAK knockout mice treated with LPS exhibited similar plasma NO levels and blood pressure when compared to the wild-type mice treated with LPS.
We found that both wild-type and SPAK knockout mice treated with LPS displayed multiple organ dysfunction syndrome.
However, no significant difference in blood flow was observed between wild-type and SPAK knockout mice treated with LPS.
Despite we use SPAK knockout mice in this experimental research, one of the major concerns is functional compensation.
SPAK applies to low wage workers who were already at work, which raises the budgetary cost of each additional job created.
The reduction in labour costs due to SPAK  Case of full-time adult workers earning the legal minimum wage, 1999 euros Without SPAK With SPAK Workers' net income 10 177 10 177 Employees' wedge 3 745 3 745 Workers' gross income (wage cost) 13 922 13 922 Employers' wedge 3 117 1 311 Labour cost 17 040 15 233 SPAK (euro 1 806) as a per cent of labour costs 10.