They then compared the genomes of affected (abnormally developed) versus unaffected (normally developed, or "healthy") zebrafish and discovered an association with the gene Sox7
, which controls how the body forms in vertebrate animals.
A list of the forward and reverse primers (targeting a SNP just upstream of sox7, which had the smallest GWAS p-value) used for the experiment can be found in Table S1 (Integrated DNA Technologies).
Expression of target gene sox7 and housekeeping gene beta actin were measured as threshold cycle ([C.sub.t]) value using relative standard curves to optimize how much input was in the reaction.
The SNPs (ranked in order of ascending p-value) were mapped to genic regions of sox7, erf, and cfap74.
Knocking down Gata4 in mESC during cardiomyogenesis led to decreased expression of Sox7 and heart muscle cell differentiation .
Hoppler, "Genome-wide transcriptomics analysis identifies sox7 and sox18 as specifically regulated by gata4 in cardiomyogenesis," Developmental Biology, vol.
Tanaka T, Wai C, et al.: Suppression of SOX7
by DNA methylation and its
SOX7 is associated with the suppression of human glioma .
Tian et al., "SOX7 is associated with the suppression of human glioma by HMG-box dependent regulation of Wnt/[beta]-catenin signaling," Cancer Letters, vol.
The PrE consists of extraembryonic endoderm precursor cells characterized by Sox7
expression that subsequently differentiate into visceral endoderm and parietal endoderm (9).
Gene deletion of EED, another component of PRC2 complex required for EZH2-mediated H3K27me3, has been reported to induce pancytopenia and increased apoptosis via derepression of p16 and p19 tumor suppressors as well as several EZH2 target genes HoxC4, Id2, Sox7