Clinical significance of chromosome 1p/19q loss of heterozygosity and Sox17
expression in oligodendrogliomas.
Deficiency of endothelium-specific transcription factor sox17
induces intracranial aneurysm.
is a critical specifier of human primordial germ cell fate.
promoter methylation in circulating tumor cells and matched cell-free DNA isolated from plasma of patients with breast cancer.
The 14 hypermethylated genes, including ADRA1D, AJAP1, COL6A2, EDN3, EPO, HS3ST2, MAGI2, POU4F3, PTGDR, SOX8, SOX17
, ST6GAL2, SYT9, and ZNF614, are implicated in [beta]-catenin signaling in cervical carcinogenesis .
The following markers were used to determine differentiation efficiency: brachyury (R&D Systems) for mesoendoderm, Nkx2.5 (Abcam) for cardiac mesoderm, Nkx2.5 and Isl1 (Developmental Studies Hybridoma Bank) for cardiac progenitors, cTnT (Thermo Fisher Scientific) for cardiomyocytes, double staining of FOXA2 (GeneTex) and SOX17
(GeneTex) for endoderm, CDX2 (GeneTex) for mid/hindgut, Otx2 (R&D Systems) for day 5 neural induction, and PAX6 (GeneTex) for neuroepithelium [17-20].
PGC specification in humans occurs at ~2 weeks after fertilization when BMP signals target to the mesendoderm of the preprimitive streak embryo, which leads to the induction of SOX17
which, with BLIMP1, suppresses endodermal differentiation and activates PGC differentiation .
The team discovered that the progenitors could be converted into blood vessel cells or into red blood cells, depending on the level of a gene transcription factor called SOX17
Wang et al., "Abnormal methylation of the sex-determining region Y-box 17 (SOX17
) promoter predicts poor prognosis in myelodysplastic syndrome," Clinical Laboratory, vol.
However, at the same time point a nuclear expression of the definitive endoderm marker protein SOX17
, a transcription factor important in the developmental process between endoderm formation and early gut formation, was detected.
This list includes ADHFE1, ALX4, CNRIP1, EID3, ELMO1, ESR1, FBN1, HLTF, LAMM, NEUROG1, NGFR, RARB, RXRG, RYR2, SDC2, SEPT9, SFRP2, SOCS3, SOX17
, THBD, TMEFF2, UCHL1, and VIM genes.
DE markers FOXA2, SOX17
and CXCR4 and also pluripotency marker OCT4 were evaluated using qRT-PCR, as well as FOXA2 by the immunocytochemistry.