SOCS-1 could inhibit the differentiation of the IFN-[gamma]-expression in cells or terminate IFN-[gamma] signal transduction to block the signaling .
Therefore, this study mainly investigated the effect of vitamin B6 deficiency on the composition and functional potential of T cell populations through analysis of pyridoxine 5'-phosphate (PLP) and xanthurenic acid (XA) plasma level, lymphocyte proliferation and differentiation, cytokines expression, and SOCS-1 and T-bet transcription.
Analysis of SOCS-1 and T-bet Gene Transcriptional Level by Real-Time PCR.
Down- and Upregulation of Gene SOCS-1 and T-bet Associated with JAK/STAT Signaling Pathway.
We also found that resveratrol induced the expression of the SOCS-1 protein and mRNA.
Conclusion: Our results suggest that resveratrol blocks STAT3 signaling pathway through induction of SOCS-1, thus attenuating STAT3 phosphorylation and proliferation in SCCHN cells.
There are seven SOCS family proteins: SOCS-1, SOCS-2, SOCS-3, SOCS-4, SOCS-5, SOCS-6, and SOCS-7, each of which has a central SH2 domain, an amino-terminal domain of variable length and sequence, and a carboxy-terminal 40-amino-acid module known as the SOCS box (Tamiya et al.
Although resveratrol has been found to mitigate aberrant activation of STAT3 in various cell lines, our study is the first one to explore the effects of resveratrol both on STAT3 signaling cascades and on the negative regulators of phosphorylated STAT3 (SOCS-1) in HNSCC cells.
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Schmiedlechner et al., "IL-4 and IL-13 induce SOCS-1 gene expression in A549 cells by three functional STAT6-binding motifs located upstream of the transcription initiation site," Journal of Immunology, vol.
Kawazoe et al., "Signals transducers and activators of transcription (STAT)-induced STAT inhibitor-1 (SSI-1)/suppressor of cytokine signaling-1 (SOCS-1) suppresses tumor necrosis factor a-induced cell death in fibroblasts," Proceedings of the National Academy of Sciences of the United States of America, vol.
Carofiglio, "Neuroprotective effects of resveratrol in an MPTP mouse model of Parkinson's-like disease: possible role of SOCS-1
in reducingpro-inflammatory responses," Innate Immunity, 2013.