The following genes were studied: b1 adrenergic receptor (Adrbl), b2 adrenergic receptor (Adrb2), glucose transporter 3 (Slc2a3), phosphorylase glycogen b (Pygb), brain-derived neurotrophic factor (Bdnf), and nerve growth factor (Ngf).
Decreased Slc2a3 expression in the amygdala but not in the hippocampus of [[beta].sub.3]-ARKO mice
No difference was found for Slc2a3, Pygb, Bdnf, and Ngf mRNA levels in the hippocampus (Figure 4A to C).
We also showed that this could be due to the decreased gene expression of Slc2a3 in the amygdala.
The present RNA-Seq data demonstrated that E2 upregulated the glycolysis enzymes glucose6-phosphatase catalytic subunit (G6PC, GeneID 2538) and hexokinase 1 (HK1, GeneID 3098), as well as a facilitated glucose transporter solute carrier family 2 member 3 (SLC2A3
, GeneID 6515), indicating increased glucose utilization and transport into cells to maintain energy production.
Wang et al., "Decreased miR-106a inhibits glioma cell glucose uptake and proliferation by targeting SLC2A3
in GBM," BMC Cancer, vol.
The presence of ammonia in culture medium results in a reduction of intracellular pH, depression of oxidative phosphorylation (Lane et al., 2002), decreased blastocyst cell numbers (Gardner and Lane, 1993), increased cellular apoptosis, perturbation of Slc2a3
expression and glucose uptake (Zander et al., 2006), and eventually altered fetal development and growth rates (Sinclair et al., 1998) as well as fetal exencephaly (Lane and Gardner, 1994).