constitutes the JH 3 and part of JH 4 regions as shown in figure 2.4,6,7,9 Although the exact function of SH2 domain
is not clear, some studies suggest that it acts as scaffolds.
Simple modular architecture research tool (SMART, http://smart.embl-heidelberg.de/) analysis showed that pCISH contained a SH2 domain
(80-169AA), a SOCS domain (210-251AA) and a SOCS_box domain (216-250AA).
Stability and peptide binding specificity of Btk SH2 domain
: molecular basis for X-linked agammaglobulinemia.
As shown in Figure 2, STAT proteins have a structural homology with a conserved carboxy-terminus SH2 domain
, a central DNA-binding domain, and an aminoterminus oligomerization domain [4, 17-19].
These domains are connected with two interdomains: interdomain A between two SH2 domains
and interdomain B between C-terminus SH2 domain
and a kinase domain (Figure 1(a)) [16-18].
The third pathway, the MAP-kinase dependent, starts when Grb2 attaches Shc or IRS to the SH2 domain
of the Grb2 protein, which is attached to Son of Sevenless (SOS), a small nucleotide exchange protein that catalyzes guanosine di-phosphate (GDP) exchange by guanosine tri-phosphate (GTP) in the protein found in the internal surface of the plasma membrane called Ras--such also favors Raf phosphorylation in serine/threonine sites.
mDab1, when tyrosine phosphorylated, binds to the SH2 domain
of Src, Abl and Fyn.
Throughout 2001, AxCell made continued progress in mapping PDZ, SH3, and SH2 domain
Because a GST fusion protein containing an SH2 domain
of Fyn can bind to PECAM-1 but an SH3 domain of Fyn cannot, the SH2 domain
of cSrc appears to be involved in c-Src binding to tyrosine-phosphorylated PECAM-1.
JAK2 has no SH2 domain
(binding phosphorylated tyrosine residue) or SH3 domain (binding proline-rich domain).
(49) The CagA-SHP2 complex formation is mediated via the interaction between tyrosine-phosphorylated EPIYA-C or EPIYA-D segment and the SH2 domains
The GM domain region has a sequence homology from all Grb7 family proteins that contains a pleckstrin homology (PH) domain, a Ras-association domain and a functional region called BPS, which is localized between the PH and SH2 domains
. The high sequence homology and the conservative molecular architecture among the Grb7 family members allow them to participate in multiple cellular signal transduction pathways (HAN et al., 2001; HOLT & SIDDLE, 2005).