Next-generation sequencing (NGS) was available in seven patients using a custom-designed 49 genes' panel (Ion S5™ System, Thermo Fisher, San Diego, CA, USA), including the entire coding region of ASXL1, ASXL2, BCOR, BCORL1, BIRC3, BRAF, CALR, CBL, CDKN2A, CSF3R, CSMD1, DNMT3A, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA2, IDH1, IDH2, IL7R, JAK1, JAK2, JAK3, KIT, KRAS, MPL, MYD88, NOTCH1, NRAS, PAX5, PDGFRA, PDGFRB, PHF6, PIGA, PTEN, PTPN11, RUNX1, SETBP1, SETD2
, SF3B1, SH2B3, SRSF2, STAG2, TET2, TP53, U2AF1, WT1 , and ZRSR2 , with a median depth of x2000.
These genes are involved in the regulation of development and somatic growth (la IGF1RB and lb IGF1RB, IGF-I receptor subtype A) , response to the DNA damage and regulation of the DNA mismatch repair (SETD2
, RIF1) [27, 28], actin filament polymerization (FH1/FH2 domain-containing protein 3, (FHOD3)), transcriptional regulation (male-specific lethal 3-like 1 (MS3L1)), and formation of centromere/kinetochore complex (centromere protein F (CENPF))  (Table 2, Supplementary File Data).
Analysis of protein-protein interaction: STRING prediction indicated that UTY interacts with heat shock protein 90 kDa alpha, class A member 1 (HSP90AA1); heat shock protein 90 kDa alpha, class B member 1 (HSP90AB1); ubiquitin specific peptidase 9, Y-linked (USP9Y); lysine (K)-specific demethylase 5D (KDM5D); histone deacetylase 2 (HDAC2); histone deacetylase 1 (HDAC1); histone deacetylase 3 (HDAC3); SET domain containing 2 (SETD2
); DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y) and sex determining region Y (SRY) (Figure 4).
Deleterious mutations have been observed to be enriched in genes that were not previously appreciated to have a role in suppressing cancer, such as the chromatin regulators ARID 1A, PBRM1, and SETD2
, thus broadening our understanding of the cellular pathways deregulated during oncogenesis.