Using DAVID, we found that hypermethylated genes in ESC cells are mostly enriched with the regulation of cell cycle (FZR1, E2F5, BOP1, TRRAP, CDK4, JUNB, etc.), cell death (SIVA1, MCL1, YPEL3, ARF6, UBQLN1, SHF, CIAPIN1, APLP1, GPX1, CASP3, etc.), and mRNA metabolic process (SCAF1
, FIP1L1, STRAP, RBM15B, CWC15, XAB2, YBX1, AUH, SF3B2, APLP1, HNRNPL, etc.); the hypomethylated genes are enriched with functions related to ATP synthesis (ATP6V1F, ATP6V1C1, ATP6V0C, ATP6V1A, ATP6V0E, ATP6V1E1, ATP5C1, etc.) and mitochondrial ribosome (MRPL15, MRPL27, MRPL16, MRPL36, MRPL39, MRPL34, DAP3, etc.) (Excel Sheet S2).
Several data show that the absence of functional supercomplex assembly factor I (SCAF1) may be involved in distribution of complex IV [74, 75, 88].
Abbreviations ANT: Adenine nucleotide translocator CAT: Carboxyatractyloside COX: Cytochrome c oxidase FCC: Flux control coefficient HBC: Human breast cancer HCC: Human colorectal cancer HK: Hexokinase MCA: Metabolic control analysis MI: Mitochondrial interactosome MOM: Mitochondrial outer membrane MtCK: Mitochondrial creatine kinase mtPTP: Mitochondrial permeability transition pore OXPHOS: Oxidative phosphorylation RC: Respiratory chain SCAF1: Supercomplex assembly factor I TME: Tumor microenvironment VDAC: Voltage-dependent anion channel.