For the evaluation of S1PR4 and S1PR5 mRNA expression, double amount of cDNA (4 [micro]l) was used and 40 cycles of amplification performed.
First, we analysed by reverse transcription and real-time PCR the expression of the five S1P receptor (S1PR) subtypes and we found that BM-MSCs expressed three of five S1PRs, S1PR1, S1PR2, and S1PR3 (Figures 1(a) and 1(b)), whereas in this experimental conditions, S1PR4 and S1PR5 subtypes were not detectable.
mRNA were determined by RT of total RNA (1 [micro]g) obtained from BM-MSCs at low--(L-) and high--(H-) density culture and 2 [micro]l of cDNA (for S1PR1, S1PR2, and S1PR3 detection) or 4 [micro]l of cDNA (for S1PR4 and S1PR5 detection) were amplified as described in Section 2.
is particularly expressed by immune cells and may therefore be critically involved in immunomodulation by S1P.
[Source:RefSeq mRNA;Acc:NM_214034] ENSSSCG00000013 474 S1PR4
sphingosine-1-phosphate receptor 4 [Source:HGNC Symbol;Acc:3170] ENSSSCG00000022 289 PCDH1 protocadherin 1 [Source:HGNC Symbol;Acc:8655] ENSSSCG00000014 427 FBXO38 F-box protein 38 [Source:HGNC Symbol;Acc:28844] ENSSSCG00000007 541 PDGFA platelet-derived growth factor alpha polypeptide [Source:HGNC Symbol;Acc:8799] ENSSSCG00000007 580 WIPI2 Sus scrofa WD repeat domain, phosphoinositide interacting 2 (WIPI2), mRNA.
Weigert, "Beyond immune cell migration: the emerging role of the sphingosine-1-phosphate receptor S1PR4
as a modulator of innate immune cell activation," Mediators of inflammation, vol.
stimulates the ERK1/2 pathway via a HER2-dependent mechanism in ER-negative MDAMB-453 breast cancer cells .
FTY720-phosphate functions as a noncompetitive inhibitor of various S1PRs [30,31], such as S1PR1, S1PR3, S1PR4
, and S1PR5, but not S1PR2, receptors [27, 32, 33].
In ER-negative breast cancer, SphK1 and S1PR4
are associated to promote tumorigenesis.
Ringleb et al., "S1PR4
signaling attenuates ILT 7 internalization to limit IFN-a production by human plasmacytoid dendritic cells," Journal of Immunology, vol.
While S1PR1, 2 and 3 are expressed ubiquitously, S1PR4 and 5 show tissue-specific distribution.
Compared to the well-characterized role of S1PR1 in these processes, the function of S1PR4 is so far underappreciated.