References in periodicals archive ?
In pigs, we have shown that uterine endometrial TRPV6 and S100G are expressed in the uterine endometrium in a pregnancy status- and stagespecific manner during the estrous cycle and pregnancy in pigs (Choi et al.
2009), but effect of estrogen on endometrial S100G expression has been not determined.
Therefore, this study was to determine i) regulation of the uterine endometrial S100G expression by estrogen and IL1B during the implantation period; ii) expression of S100G in conceptuses during early pregnancy; and iii) expression of S100G in the uterine endometrium from gilts with SCNT-derived conceptuses compared to that from gilts with conceptuses derived from natural mating on day (D) 12 of pregnancy.
To determine the effects of cytokines on S100G expression, explant tissues were treated with 0, 1, 10, 100 ng/ml IL1B (catalog number 19401; Sigma) in the presence of both [E.
Total RNA extraction, cloning of porcine S100G and RT-PCR
The cDNA templates were then diluted 1:4 with sterile water and amplified by PCR using Taq polymerase (Takara Bio, Shiga, Japan) and specific primers (forward, 5'-TCC TGC AGA ACT GAA GAG CA-3'; reverse, 5'-GCA GAG ACA TGG GTG GTT TT-3') based on mRNA sequences of porcine S100G (GenBank accession no.
To analyze levels of S100G mRNAs in the uterine endometrium, real-time RT-PCR was performed using the Applied Biosystems StepOnePlus System (Applied Biosystems, Foster City, CA, USA) using the SYBR Green method.
Data from real-time RT-PCR analysis for S100G mRNA levels were subjected to least squares ANOVA using the General Linear Models procedures of SAS (Cary, NC, USA).
Regulation of S100G expression by steroid hormones in the uterine endometrium pregnancy in pigs
To determine whether endometrial S100G expression was affected by steroid hormones and during the implantation period in pigs, the uterine endometrial tissue explants from gilts on D12 of the estrous cycle were treated with [P.
Effect of IL1B on S100G expression in the uterine endometrium on in pigs
2003), on S100G expression, we performed real-time RT-PCR analysis using the uterine endometrial explant tissues obtained on D12 of the estrous cycle and treated with 0, 1, 10, or 100 ng/ml IL1B in the presence of both [E.
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