6) Although diluted Russell viper venom time and activated partial thromboplastin time (aPTT) sensitized with silica and low phospholipid concentration are primarily recommended, diluted prothrombin time (dPT) using diluted Innovin reagent (human recombinant thromboplastin; Siemens, Marburg, Germany) also provides an inexpensive assay with high sensitivity and high negative predictive value.
12,13) In previous studies, prolongation of the dPT was found to be associated with higher risk of thrombosis, (14) and receiver operating characteristic curve analysis indicated that the dPT ratio might be more useful for predicting thrombosis than diluted Russell viper venom time.
In vitro experiments have demonstrated that concentrations of DTIs in plasma within the therapeutic range could potentially modify the results and interpretation of diluted Russell viper venom time and LA-sensitive aPTT tests, whereas fondaparinux does not affect diluted Russell viper venom time.
LAC was detected again on admission with the following results: kaolin clotting time greater than 200 seconds (normal range 100 to 160), dilute Russell Viper Venom
Time 96 seconds (normal range 0 to 45), Russell Viper Venom
+Phospholipid Time 50 seconds (normal range 0 to 38) with a resultant dilute Russell Viper Venom
/Russell Viper Venom
+Phospholipid Time index of 1.
The effect is more pronounced with reagents whose phospholipid content is relatively low (12) and may be greater with the kaolin clotting time (KCT) than with the dilute Russell viper venom test (dRVVT) (14).
The use of the dilute Russell viper venom time for the diagnosis of lupus anticoagulants.
Kaolin clotting time and dilute Russell viper venom time distinguish between prothromb-independent and beta 2-glycoprotein I-dependent anti phospholipid antibodies.
Heterogeneity of Russell viper venom affects the sensitivity of the dilute Russell viper venom time to lupus anticoagulants.
The importance of locally derived reference ranges and standardized calculation of dilute Russell viper venom time re sults in screening for lupus anticoagulant.
Laboratory diagnosis of lupus anticoagulants for patients on oral anticoagulant treatment: performance of dilute Russell viper venom test and silica clotting time in comparison with Staclot LA.
09, and the presence of lupus anticoagulant (LA) antibodies (prolonged Russell viper venom
time and confirmed by the STACLOT LA ELISA test, Reaads Medical Products, Inc.
LA detection was performed on filtered plasmas according to the criteria of the Scientific and Standardization Committee (SSC) of the International Society on Thrombosis and Hemostasis (ISTH) (9) with screening and confirmatory procedures carried out with 3 tests: home-made silica clotting time (SCT), home-made and commercial dilute Russell viper venom
tests (dRVVT), and an activated partial thromboplastin time (APTT) with hexagonal phospholipids.