Moreover, application of internal ribosomal entry site (IRES) system for dual expression of two transgenes, which are transcribed under a unique promoter and using an IRES as a spacer and ribosomal binding site
, has been widely documented to investigate the cellular transfection efficiency by evaluation of enhanced green fluorescent protein (EGFP).
2002), and constituted the ribosomal binding site
for mRNA in the 40S initiation complex (Takahashi et al.
Ribosomal binding site (RBS) and His*Tag and S*Tag sequences were added between light and heavy chain sequences, upstream of heavy sequence.
Each expression cassette contains the following components: 1-A T7 promoter, located on pET-32 Ek-LIC which controls transcription of light and heavy chain genes; 2-Two Ribosomal Binding Sites (RBS) preceded each chain.
The ribosomal binding site which is located directly after the transcription initiation site and before the translation start codon is used to help in locating the start codon.
In many bacteria the ribosomal binding site has a consensus motif of AGGAGGT.
Linezolid, a member of the new oxazolidinone class of drugs, is an expensive treatment option that is active at the ribosomal binding site
of the bacterial cell.
Alteration of the ribosomal binding site
is an important mechanism by which bacteria become resistant to antibiotics.
Some of these regions are located in ribosomal binding sites
as previously reported .