Xenogeneic, extracellular cytotoxic
ribonucleases (RNases) of bacterial or eukaryotic origin, that trigger apoptosis/ cytotoxicity in tumor and other cells, may have a great potential as anticancer drugs [7-11].
The nuclear RNase III endonuclease Drosha, the cytoplasmic RNase III
ribonuclease Dicer, and the microprocessor component DGCR8 are essential for miRNA maturation [35].
The
ribonuclease activity of binase was registered by 260 nm absorbance measuring of the products of binase hydrolysis of high molecular weight yeast RNA.
The [sup.3]H-labeled 3'-terminal nucleoside was cleaved by
ribonuclease and was analyzed by two dimensional paper chromatography that can identify nucleoside derivatives (A', C', G' and U') with modified ribose residues.
RNA extraction is complicated because of the presence of
ribonuclease enzymes in cells and tissues that can rapidly degrade RNA.
Kyogoku, "Identification of two essential histidine residues of
ribonuclease T2 from Aspergillus oryzae," European Journal of Biochemistry, vol.
Aspergillus niger ATCC26550 the source material for
ribonuclease production was obtained from NCL.
Isolation of a novel ubiquitin-like protein from Pleurotus ostreatus mushroom with anti-human immunodeficiency virus, translation-inhibitory, and
ribonuclease activities.
Gilliland, The conformational flexibility of surface residues of bovine
ribonuclease A at 1.1 [Angstrom] resolution, in Structure, Mechanism and Function of
Ribonucleases, C.
(1995) estimated and then synthesized ancestral amino acid sequences of artiodactyl pancreatic
ribonuclease. The enzyme is present in large quantities in the guts of ruminants and species with ruminant-like digestion, where it digests RNA derived from symbiotic microflora (Beintema et al.
The first step, a phenol/chloroform extraction performed at 0 [degrees] C, was crucial for the removal of
ribonuclease activity, and the second step, employing ultracentrifugation through a cesium chloride gradient, removed an inhibitor of reverse transcriptase.
