Fourier Transform Infrared (FT-IR) spectra, to observe impregnation of Rhodamine B into silica pores, were acquired within the 4000 to 500 [cm.
The SAXS measures in the Figure 3, were performed in order to collect information about structure and arrangement of pores, the spectra of silica impregnated with Rhodamine B in different pH values are shown below.
1], assigned ascribed to the C-H stretching modes that might be attributed to aromatic rings of Rhodamine B .
A significant shift occurs for silica impregnated with Rhodamine B solution in pH 11, indicating changes must be related to removing dimers when increase the pH, leading to the observation of monomer emission , consequently influences the spectroscopic properties of Rhodamine B, which also observed for the others techniques used.
Results show that, in acidic medium, Rhodamine B impregnated in silica has a stable behavior.
The transporter activity assay was performed with 1 [micro]M rhodamine B as described above.
After exposure to rhodamine B and test compounds, the dye was extracted from the biopsy tissue disks in a defined volume of solvent, and the amount of dye was then measured fluorometrically.
Furthermore, we found that transporter activity, indicated by the amount of rhodamine B accumulated by the tissue, was consistent among tissue disks from the same individual (e.
In control tissues, low basal efflux transporter activity is indicated by high accumulation of rhodamine B, whereas high basal efflux activity is indicated by low levels of rhodamine fluorescence (because more dye is effluxed from the cells).
Efflux transporter activity tended to slightly increase over time, as indicated by the lower amounts of rhodamine B in control tissues.