reverse transcriptase polymerase chain reaction

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reverse transcriptase polymerase chain reaction (RT-PCR),

a process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.

re·verse trans·crip·tase pol·y·mer·ase chain re·ac·tion

(RT-PCR) (rē-vĕrs' tran-skrip'tās pol-im'ĕr-ās chān rē-ak'shŭn)
A process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.
References in periodicals archive ?
Reverse transcription polymerase chain reaction and real time-quantitative polymerase chain reaction
Of the 22 patients receiving casimersen who have been tested for increased exon-skipping mRNA using reverse transcription polymerase chain reaction, all have displayed an increase in skipping exon 45 over their baseline levels, representing a 100% response rate.
Researchers developed a real-time reverse transcription polymerase chain reaction test for a mutation of codon 91 in the gyrase A (gyrA)gene in N.gonorrhoeae that reliably predicts resistance to ciprofloxacin.
This solution is configurable and expandable, offering a sample-to-knowledge workflow for next generation sequencing (NGS), reverse transcription polymerase chain reaction (RT-PCR) and capillary electrophoresis (CE) sequencing applications.
Xpert Xpress Flu utilises automated real-time reverse transcription polymerase chain reaction (RT-PCR) to detect Influenza A and B RNA.
At UCLA, researchers developed a real-time reverse transcription polymerase chain reaction test for a mutation of codon 91 in the gyrase A (gyrA) gene in N.
In the detection process, the application of reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay play an important role.
Methods: Reverse transcription polymerase chain reaction and immunohistochemistry were used to determine the expression of Hsulf-1 in HCC.
Oropharyngeal and cloacal swabs tested positive for influenza A virus H5N8 by real-time reverse transcription polymerase chain reaction. The virus was subsequently isolated.
(4) Subsequent serological and molecular work confirmed this finding in camels connected to human cases in Qatar, which were positive for MERS-CoV by real time reverse transcription polymerase chain reaction (rRT-PCR) from nasal swabs.
The test measures the body's genetic response to viral infection using a reverse transcription polymerase chain reaction (RT-PCR) TaqMan low-density array (TLDA) platform.
ABBREVIATIONS: bDNA = Branched DNA; CD = Cluster of Differentiation; CI = Confidence Interval; CIN = Cervical Intraepithelial Neoplasia; DNA = Deoxyribonucleic Acid; FDA = Food and Drug Administration; FRET = Fluorescence Resonance Energy Transfer; HBV = Hepatitis B Virus; HCV = Hepatitis C Virus; HIV = Human Immunodeficiency Virus; HPA = Hybridization Protection Assay; NASBA = Nucleic Acid Sequence Based Amplification; PCR = Polymerase Chain Reaction; QS = Quantitation Standard; RLU/CO = Relative Light Units/Cutoff Value; RNA = Ribonucleic Acid; RT = Reverse Transcription; RT-PCR = Reverse Transcription Polymerase Chain Reaction; Taq = Thermus aquaticus; TMA = Transcription-Mediated Amplification.

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