reverse genetics


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reverse genetics

a conceptual approach allowing the study of gene function; it can be summarized as "from gene to phenotype" as opposed to forward genetics, which can be summarized as "from phenotype to gene." It uses structural changes in the gene by deletion through gene knockout or disruption by site-directed mutagenesis, and then investigating the effects of such alterations on the phenotype of the cell or organism.
Synonym(s): positional cloning
References in periodicals archive ?
Approaches such as reverse genetics enable the researchers to delve into the complexities of the virus's disease-causing machinery and its ability to mutate and "pilfer" genetic material from its hosts, potentially adding to its virulence.
With the help of polygonal illustrations, prediction can be done in either direction as reverse genetics (genotype to phenotype prediction) or forward genetics (phenotype to genotype).
(65) To elucidate all the gene functions in a model organism will require integrated genetics, which is a combination of forward and reverse genetics, a systematic and large set of reduction-of-function studies using RNAi clones (73) or gene knockout strains, which are or will be available for C.
H1N1 LAIV reassortant viruses, with a 6 : 2 or 5 : 3 genome composition, were generated by standard plasmid-based reverse genetics, as described elsewhere [13].
Protective efficacy in chickens, geese and ducks of an H5N1-inactivated vaccine developed by reverse genetics. Virol., 341: 153-162.
Scientists first documented the cancer-fighting properties of Newcastle disease virus in the 1950s, but it is only with recent advances in reverse genetics technology that they have turned to the genetically engineered virus as a possible treatment.
Reverse genetics of RNA viruses; applications and perspectives.
compile 12 articles by researchers in virology, microbiology, infectious diseases, immunology, and related fields in North America, Europe, New Zealand, and Australia who detail current research findings on caliciviruses and related diseases, including norovirus epidemiology, calicivirus contamination of the environment, genome organization and recombination, proteolytic cleavage and viral proteins, viral protein structures, virus-host interactions, reverse genetics and replicon systems, feline calicivirus, murine noroviruses, and lagoviruses.
A technique called reverse genetics was used to create a hybrid between the H1N1 swine flu virus and a tested laboratory strain.
* Reverse genetics (testing whether candidate genes and polymorphisms identified in animal studies as relevant for biological effects also exist in humans and are relevant to the phenotype).
For example, in response to the avian influenza outbreaks throughout the world, FDAH utilized reverse genetics, a cuttingedge technology being used in the development of human vaccines.
(Gaithersburg, MD) licensed its proprietary reverse genetics intellectual property to GlaxoSmithKline to support the development and construction of new vaccine strains to produce non-live human influenza vaccines.