reverse transcriptase polymerase chain reaction


Also found in: Acronyms.

reverse transcriptase polymerase chain reaction (RT-PCR),

a process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.

re·verse trans·crip·tase pol·y·mer·ase chain re·ac·tion

(RT-PCR) (rē-vĕrs' tran-skrip'tās pol-im'ĕr-ās chān rē-ak'shŭn)
A process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.
References in periodicals archive ?
5% of cases were diagnosed when combining histopathological examination, BACTEC culture and reverse transcriptase polymerase chain reaction on pleural biopsy specimen.
Preoperative combined nested reverse transcriptase polymerase chain reaction for prostate-specific antigen and prostate-specific membrane antigen does not correlate with pathologic stage or biochemical failure in patients with localized prostate cancer undergoing radical prostatectomy.
2% (9) were found to have HMPV by reverse transcriptase polymerase chain reaction (RT-PCR) technology (Emerg.
The level of enzyme activity was found to correlate exactly with the concentration of the enzyme, as measured by both Western blot and reverse transcriptase polymerase chain reaction tests.
Using both immunoassay and reverse transcriptase polymerase chain reaction (RT-PCR) methods, the Company's scientists are continuing to refine the methods for detecting the NMP66 complex in the blood of women with breast cancer.
Choanal and/or cloacal swabs were obtained and submitted to Virginia's Division of Consolidated Laboratory Services (Richmond, VA, USA) for analysis with real-time reverse transcriptase polymerase chain reaction (RT-PCR).
Comparison of Vero cell plaque assay, TaqMan reverse transcriptase polymerase chain reaction RNA assay, and VecYest antigen assay for detection of West Nile virus in field-collected mosquitos.
Tafra analyzed 38 false-negative biopsy specimens taken from patients using reverse transcriptase polymerase chain reaction for mammoglobin and detected tumor cells in 55% of the nodes.
1] Nonstandard abbreviations: RT-PCR, reverse transcriptase-PCR; EGFR, epidermal growth factor receptor; and CURT-PCR, calibrated user-friendly reverse transcriptase polymerase chain reaction.
The study includes results compared with culture and the most sensitive diagnostic technique available in any clinical laboratory -- the reverse transcriptase polymerase chain reaction (RT PCR).
Sequences were obtained from reverse transcriptase polymerase chain reaction (RT-PCR)--amplified portions of the nucleoprotein (positions 1,157-1,477), based on the reference strain M13215 (17).
Reverse transcriptase polymerase chain reaction (RT-PCR) [3] provides highly sensitive detection of mRNA sequences and has been used widely for the study of gene expression in malignant and physiological states (5, 6).

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