The bone marrow suppressive activity of these aromatic compounds was assessed indirectly through the reticulocyte count and reticulocyte index
, which measures the red cell output from the bone marrow .
Regarding blood cell counts, there was a borderline decrease in the erythrocyte count and a significant increase in the reticulocyte index
in the EOPE group when compared to the C group.
Hemoglobin was 8.1 g/dL, with reticulocyte index
We demonstrated that analysis of the reticulocyte count or reticulocyte index
could not distinguish anemic causes with reduced erythropoietic activity in our cohort of patients (see Fig.
Her white cell count was 2.5 (4.5-11 x 103/uL); absolute neutrophil count 449; haemoglobin (Hb) 6 (12-16 g/dL); MCV 100 (82-101 fl); platelet 7 (140-440 thou/cumm); reticulocyte count 0.9% (0.5-1.5%); reticulated haemoglobin 44.7 (28.8-37.7 pg); reticulocyte index
0.8%; vitamin B12 94 (211-946 pg/mL); intrinsic factor antibody 11.9 (0-1.1 AU/mL); creatinine 1.07 (0.5-0.95 mg/dL), and serum protein electrophoresis showed a monoclonal spike 3.13 g/dL in the gamma region corresponding to IgG lambda paraprotein.
Furthermore, in the background of macrocytic anemia and a reticulocyte index
of 1.04 at presentation, which worsened to Hb of 6.9 mg/dL and Hct of 20.6% on the third day of presentation, the initial serum Vitamin B12 returned to low level (202 pg/mL, normal 211-946 pg/mL) with normal serum folate (5.9 ng/mL, normal > 3.0 ng/mL) in the presence of anti-intrinsic factor (IF) antibody.
He had a normal haptoglobin, LDH, reticulocyte index
, and fibrinogen.
Crocin at all doses (50, 100 and 200 mg/kg) and safranal at lower doses (0.025 and 0.05 ml/kg) decreased the higher reticulocyte index
induced by diazinon.
To clarify the differential diagnoses, additional studies could have been evaluated at this point: a C-reactive prorein to define the presence of an inflammatory process; an intact parathyroid hormone (PTH) level to rule out secondary hyperparathyroidism; and serum lactate dehydrogenase (LDH), absolute reticulocyte count, reticulocyte index
, and reticulocyte production index as markers of erythropoieric activity.
Q When doing a reticulocyte count on a newborn, if the hematocrit is greater than 0.45 but the polychromasia is moderate or marked, should a reticulocyte index
still be performed?
On the 9th day of hospitalization the laboratory tests showed: Hb 8 gr/dl; WBC 1.5 X [10.sup.9]/l (neutrophils 0.1 X [10.sup.9]/l, lymphocytes 1.2 X [10.sup.9]/l, monocytes 0.2 X [10.sup.9]/l); platelet count 19 X [10.sup.9]/l (normal-sized platelets in peripheral blood smear); corrected reticulocyte index
0.6% (absolute reticulocyte count 4 X [10.sup.9]/l).
In conclusion, the reticulocyte channel of the Sysmex XE-2100 series offers a new reticulocyte index
, the Ret-Y, which shows good correlation with the CHr.