Alignment of whole-genome restriction maps and in silico map for Bartonella isolates from patients with verruga peruana, rural Ancash region, Peru.
For whole-genome restriction map (WGRM) analysis, Bartonella isolates were freshly grown on brain heart infusion agar with 10% sheep blood at 30[degrees]C in an atmosphere of 5% C[O.sub.2] for 10-14 days.
The Red polygon in figure (3.B) indicated the restriction for the existing dumping site at Mehmood Booti Lahore whereas figure 3 (C, D and E) depicted the restriction maps
for the roads, water bodies and tube wells, respectively.
Injectivity of the restriction maps
. We need some results regarding restriction maps
for Galois cohomology.
For similar work regarding restriction maps
, see [8, 9].
We further assume that these restriction maps
from the vector space of sections corresponding to [M.sub.i] to both [L.sub.i-1] and [L.sub.i] are isomorphisms.
of the ITS1 sequences were done using the software Primer-Premier.
of BAC clones RP22-78A 19, -44B20, -362C 15, -127H7, -548M4, -160014, and RP23-430G14, -174D7, and -113D13 were constructed using Eco RI and Hind III (data not shown).
These mutations were mapped to specific regions on either of the two restriction maps
. Using these data, gene trees depicting the most parsimonious phylogeny of the mtDNA haplotypes were empirically derived (Avise 1989).
where f, g, h are the natural restriction maps
and m the natural multiplication map.
The whole-genome restriction map
for R1101 was compared with in silico NcoI restriction maps
of 33 complete genome sequences from the family Nocardiaceae, retrieved from GenBank; these sequences included R.
Evolutionary inferences from restriction maps
of nine taxa of Xenopus frogs.