Flow cytometric analysis of neutrophil's oxidative burst using dihy-drorhodamine (DHR) showed absence of respiratory burst
in his neutrophils suggestive of CGD (fig-2).
CGD is caused by a genetic defect in the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme complex, which fails to display a characteristic increase of oxidative metabolism, called the "respiratory burst
," during phagocytosis (1).
Leukocytes respiratory burst
activity as indicator of innate immunity of pacu Piaractus mesopotamicus.
In inflammatory process, neutrophils go through a process of respiratory burst
which results in increased consumption of oxygen then undergoes reduction via NADPH oxidase to superoxide anion and harmful reactive oxygen species (ROS).
In addition, random migration and chemotaxis of neutrophils induced by fMLF was significantly reduced (Figure 2(b), mean random migration = 0.67 mm and chemotaxis = 2.26 mm, compared with 1-step mean random migration = 0.96 mm and chemotaxis = 2.64 mm), whilst baseline respiratory burst
activity raised (mean DHR-123 MFI = 252.3 compared with 1-step MFI= 193.7 and mean peak RFU = 4053, compared with 1-step peak RFU=1713).
Figure 1 shows the detected phagocytic activity of monocytes, granulocytes and respiratory burst
of phagocytes in tested blood samples after 48 h exposure.
Through the agency of the receptors being complementary to the complement components, opsonized immune complexes interact with neutrophils that undergo a respiratory burst
in the presence of zymosan.
The respiratory burst
(NBT) activity can be quantified by the Nitroblue Tetrazolium (NBT) assay, which measures the quantity of intracellular superoxide radicals produced by leukocytes (Sahu et al., 2007; Ardo et al., 2008).
prowazekii-infected macrophages also does not depend on the macrophage respiratory burst
(Turco et al.
The ALCAT measures changes in cell size and volume after incubation with foods or other substances, including the swelling of the cell, the decrease in cell numbers with degranulation of neutrophils, and the respiratory burst
, releasing activated NADPH oxidase, generating superoxide anion and hydrogen peroxide, which then give rise to reactive oxygen species.
Chronic granulomatous disease (CGD) is a relatively rare genetic disorder characterised by defect in respiratory burst
activity of phagocytes that is associated with intracellular killing of phagocytosed microorganisms.1 In CGD, the primary defect is associated with the key enzyme, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase.