donovani cell lines expressing firefly luciferase reporter gene
(luc.) have been developed as a part of episomal vector and suitability of these cell lines for in vitro screening of antileishmanial agents has been establised (52).
Luciferase Reporter Gene
Assay--Transient transfection was performed as described by the Fugene 6 manufacturer's protocol.
Fusion of the isolated promoters to the uidA reporter gene
and use of transient GUS expression assays proved an effective method of confirming each promoter's activity in sugarcane and wheat cells.
Which potential benefit is associated with multiple reporter gene
If this assumption is valid, luminescence measurements directly reflect reporter gene
(1991); additionally, the study showed that the 2.2-kb hGFAP promoter drives expression of a LacZ reporter gene
in mice in a manner similar to that of endogenous GFAP (Brenner et al., 1994).
Chen et al., "Tyrosinase as a multifunctional reporter gene
for photoacoustic/MRI/PET triple modality molecular imaging," Scientific Reports, vol.
Reagents and assay kits include reporter gene
assays, second messenger assays, cell growth assays, cell death assays and other assays.
The assay, integrating the luciferase reporter gene
technology, is an all-inclusive platform that incorporates two optimised media to be used during cell culture and in diluting test samples, a reference agonist, Luciferase Detection Reagent and a cell culture-ready assay plate.
In SIF-seq, hundreds or diousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene
and targeted into a single, reproducible site in embryonic cell genomes.
The [bla.sub.TEM-1] gene has been placed under the control of the LexA-binding box and used as reporter gene
. The expression of TEM-1 [beta]-lactamase enzyme was increased in the presence of ssDNA induced by levofloxacin, while, the presence of curcumin at 8 [micro]g/ml, reduced dramatically the expression of the reporter gene
To construct reporter plasmids, we used the pGL3-Basic vector, in which two random cis-elements, 5xGAL4 and 3xDRE, and a minimal TATA promoter  were cloned in the MCS before luciferase reporter gene
. The 35S::DBD- (GAL4-DNAbinding domain-) CMYB1 effector plasmid was constructed by inserting DNA fragments containing CMYB1 coding region and GAL4-DNA binding domain sequence of pGBKT7 vector (Clontech, USA) into the Spe I and Xba I sites of pA7.