Site-specific inversion require the presence of specific recombinase
enzymes which invert particular DNA fragment resulting in ON to OFF or OFF to ON switching of gene placed within the invertable segment of DNA or next to the switch.
However, for medical or environmental applications, the recombinases
could be re-engineered to respond to other conditions such as acidity or the presence of specific transcription factors (proteins that control gene expression).
cholerae genome depends on two host encoded tyrosine recombinases
, XerC and XerD (18).
Phase variation of the 987P-like CS18 fimbriae of human enterotoxigenic Escherichia coli is regulated by site specific recombinases
In jawed vertebrates these recombinases
mediate the rearrangement process.
An appropriate combination of these recombinases
would improve the overall ability to characterize a gene function more precisely (Raymond and Soriano, 2007).
Cozzarelli elucidated the nature of DNA architecture and growth through enzymologic studies of polymerases, ligases, recombinases
, and topoisomerases.
These include restriction endonucleases for cleaving DNA and ligases for joining the fragments, recombinases
that can execute a large variety of DNA cleavage and joining reactions.
We use the wild type strain WFD11 and its derivative, DS52, which lacks the radA gene, an archaeal member of the recA/RAD51 family of recombinases
Not only are the constructs more complicated and hence more unstable and prone to horizontal gene transfer, the gene products used are cell poisons or recombinases
, ie, genome scramblers.
Transgene Manipulation in Zebrafish by Using Recombinases
They used a class of proteins known as recombinases
, which can record information into bacterial DNA by recognizing specific DNA addresses and inverting their direction.