cholerae genome depends on two host encoded tyrosine recombinases
, XerC and XerD (18).
Talking about a revolution: the impact of site-specific recombinases
on genetic analyses in mice.
, this gateway affords its collaboration partner the unique flexibility to develop customizable biologically-based therapeutic products to better animal health from their own facilities with an integrated end-to-end solution.
Those circuits depend on enzymes known as recombinases
, which can cut out stretches of DNA, flip them, or insert them.
Examples of topics covered include the methods for homologous recombination, the use of recombinases
in gene activation/inactivation and transgenesis, immunolabeling of embryos, imaging cellular and molecular dynamics in live embryos using fluorescent proteins, imaging of neuromuscular junctions, imaging pupal wing morphogenesis, microscopic analysis of the adult Drosophila retina using semithin plastic sections, extraction and immunoblotting of proteins from embryos, mass production of embryos and chromatographic purification of native protein complexes, and flow cytometric analysis of Drosophila cells.
dagger]) Two isolates had recombinases
identical to SCCmec IVe on the basis of sequence information; 4 isolates were not typeable.
We use wildtype strain WFD 11 and its derivative, DS52, which lacks the radA gene, a member of the RAD51/recA family of recombinases
Those enzymes belong to a class called the recombinases
, which recombine specific portions of DNA.
Full coverage of the role of gene expression during development, and the new field of genetic neuroantomy using site-specific recombinases
However, the excision and integration of the mec DNA from and to the chromosome are apparently catalyzed by cassette chromosome recombinases
A and B (Ccr A and B) coded by mec-associated genes (ccrA and B), with homology to the invertase-resolvase family of DNA recombinases
Immediately prior to joining MediChem, he was an assistant professor at San Diego State University and supervised a research program studying DNA recombinases
, topoisomerases and phosphodiesterases, important protein targets in the area of cancer research and cell signaling.
Elements found within three types of mec-associated DNA mec types Genetic feature(a) I II III Size 32 kb 52 kb 60 kb mecA + + + mecR1-mecI - + + ccrAB + + + pUB110 - + - IS431 (number) 1 2 4 Tn554 (number) 0 1 2 Tc, Hg resistance - - + (a) mecA = gene encoding PBP 2a, the penicillin-binding protein with low binding affinity that mediates methicillin resistance; mecR1-mecI = sensor-transducer and repressor genes that regulate production of inducible PBP 2a; ccrAB = cassette chromosome recombinases
A and B that mobilize the mec element; pUB110 = integrated plasmid that encodes tobramycin and kanamycin resistance; IS431 = insertion sequence; Tn554 = erythromycin-resistance encoding transposon; Tc = tetracycline-resistance determinant; Hg = mercury-resistance determinant.