SPLUNC1 cDNA-ORF contains one long open reading frame
of 768 bp from 64 nt to 831 nt.
In total, the DNA-based libraries produced 1,029 unique variants in both tissues and the cDNA libraries produced 241 unique variants in both tissues across the four open reading frames
Intentional mismatches in the annealing portion of the second-stage PCR primers were engineered to mutate natural stop codons, found in nonnatural reading frames
or in intronic sequences, to create uninterrupted coding potential from an amplified fragment and permit fusion with the downstream 6xHis sequence provided by the expression vector.
Comparisons of the base sequences of the reading frames
of the two genes showed 11 base pair differences resulting in four amino acids differences (Fig.
Such functional genomics projects often rely upon the precise cloning of large numbers of open reading frames
(ORFs) into various expression vectors.
9 kb) inverted repeat containing the rRNA cistrons, 30 tRNA genes, 44 genes encoding proteins for photosynthesis, 44 ribosomal protein genes (26 large subunit and 18 small subunit), 5 genes for biosynthetic function, 19 genes involved in gene expression (including 3 hypothetical chloroplast reading frames
or ycfs), 25 additional ycfs, and 9 open reading frames
(ORFs) larger than 50 amino acids.
The fact that most of the 5'-proximal AUG sequences examined were blocked by downstream stop codons is a striking coincidence and raises the further question of what the functions of short, upstream open reading frames
might be aside from the possibility that they encode short polypeptides with as yet undiscovered biological functions.
The virion glycoproteins of Ebola virus are encoded in two reading frames
and are expressed through transcriptional editing.
Invitrogen has utilized the power of Gateway(R) Technology in building its Ultimate(TM) ORF Clone Collection -- a wide-ranging collection of full-insert sequenced human and mouse open reading frames
TBF1 protein accumulation is under a tight translational regulatory mechanism involving upstream Open Reading Frames
(uORFs) located in the 5'Untranslated Region of the TBF1 mRNA.
Nucleotide sequencing combined with a search of the GenBank database revealed five clones generated by 5'RACE-PCR ([approximately equal to] 500) (Figure 1), as having no the initiator element in their open reading frames
However, the team has noted that expression of some of these viral open reading frames
has been detected in human tissues, which supports the possibility that they are biologically active in host species.