The analysis of the glycoprotein open reading frame
(Figure 2, panel C) leads to a similar result; OKAV shares the most recent common ancestor with MWV, and MRTV clusters with IPPYV but with a weaker bootstrap support.
The open reading frame
encoded a 157 amino acid residue protein with an isoelectric point (pI) of 7.
Intentional mismatches in the annealing portion of the second-stage PCR primers were engineered to mutate natural stop codons, found in nonnatural reading frames
or in intronic sequences, to create uninterrupted coding potential from an amplified fragment and permit fusion with the downstream 6xHis sequence provided by the expression vector.
Such functional genomics projects often rely upon the precise cloning of large numbers of open reading frames
(ORFs) into various expression vectors.
Perhaps short, 5'-proximal open reading frames
provide similar attenuating functions among the examples cited in this report.
From building open reading frame
clone collections and cDNA libraries, to protein detection, purification and production, Gateway(R) plays a vital role.
However, the team has noted that expression of some of these viral open reading frames
has been detected in human tissues, which supports the possibility that they are biologically active in host species.
Sequence analysis indicated that the tentatively named raven circovirus (RaCV) was 1898 nucleotides in size with 2 major open reading frames
synonymous with other avian circoviruses, ORF C1 and ORF V1, likely to encode a putative capsid protein (Cap) and replicase-associated protein (Rep), respectively.
Isolated pieces of RNA are labeled with a fluorescent dye and then allowed to hybridize to chips, each of which is studded with up to 40 oligonucleotides complementary to each of the 6,200 specific yeast genes and open reading frames
(areas that are likely to be protein-coding regions of the genome).
Examples of these include the identification of open reading frames
encoding potential genes obtained from raw nucleotide sequence data from the Human Genome Project; and the BLAST program used to compare a nucleotide/amino acid sequence with all sequences in the GenBank database.
Yeast Oligo Microarray Kit -- this microarray provides more than 10,000 60-mer probes representing 6,256 known open reading frames
(ORFs) in the yeast genome.
pdf) to obtain the HEV genome consisting of all the 3 open reading frames