Rabbit Ileal Loop Test

A virtually retired technique used to identify enterotoxins produced by E coli and Salmonella spp, in which the supernatant from these organisms in culture is inoculated into a ligated segment of ileum, which responds by ‘locking’ the intestinal adenylate cyclase into the ‘on’ position, increasing secretion of fluids into the loop of intestine
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shigelloides in rabbit ileal loop test, where the culture filtrates caused dislodgement of CHO and mouse adrenal tissue-culture cells.
16; in the rabbit ileal loop test, an hemorrhagic mucus-like fluid was observed (25-30 mL).
The rabbit ileal loop test was carried out according to the methods described by De and Chatterjee.