RREB1

RREB1

A gene on chromosome 6p25 that encodes a zinc finger transcription factor which binds to RAS-responsive elements (RREs) of gene promoters, and may participate in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression.
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The RREB1 gene encodes a zinc finger transcription factor [14].
Additionally, the in silico mapping showed that the AFLP markers were located on SSC7, SSC8, SSC9, SSCIO, and SSC17 and were mapped close to the strong functional candidate genes for fatness traits including, RREB1, GUCY1B3, 0R51VI, ANKRD16, CYP24A1, and D0K5, respectively These findings promote the importance of all genes as the positional candidate genes for fat deposition in the muscles of pigs.
When using a 4-probe FISH panel evaluating loci commonly altered in melanoma--MYB (6q23), RREB1 (6p25), CCND1 (11q13), and centromere 6 (Cep6)--studies have found high sensitivity and specificity when distinguishing unequivocal nevi from melanoma.
More recently, an alternative FISH panel has been tested that replaces MYB and centromere 6 with CDKN2A and MYC, thus evaluating genetic alterations at RREB1 (6p25), MYC (8q24), CDKN2A (9p21), and CCND1 (11q13).
Among the regions identified by the international research team are two, near the genes ARL15 and RREB1, that also show strong links to elevated levels of insulin and glucose in the body - two key characteristics of type 2 diabetes.
Sensitivity of fluorescence in situ hybridization for melanoma diagnosis using RREB1, MYB, Cep6, and 11q13 probes in melanoma subtypes.
Per NeoGenomics Laboratories recommendations, (16) FISH was considered abnormal if at least one of the following criteria was met: (1) more than 16% of the nuclei showed more than 2 signals for RREB1 (red probe), (2) the ratio of red signal (RREB1) to aqua signal (centromere 6) greater than 1 was observed in more than 53% of the nuclei, (3) the ratio of yellow signal (MYB) to aqua signal (centromere 6) less than 1 was observed in more than 42% of the nuclei, and (4) more than 19% of the nuclei showed more than 2 signals for CCND1 (green probe).
MYB1 loss appears particularly common in spitzoid neoplasms and is rare in dysplastic nevi, whereas the opposite seems to be true for RREB1.
A commercially available FISH panel for the diagnosis of melanoma contains 4 probes directed against 2 chromosomes (6p25 RREB1, 6q23 MYB, 6centromere, 11q13 CCND1).
From this analysis, the following 4 FISH criteria were identified: (1) if more than 38% of enumerated cells contained more than 2 signals for CCND1 (11q13) or (2) if more than 55% of nuclei contained more signals for 6p25 than for centromere 6 or (3) if more than 40% of nuclei contained fewer signals for MYB (6q23) than for centromere 6 or (4) if more than 29% of cells had more than 2 RREB1 (6p25) signals, then a case would be considered as positive for melanoma (18) (Table).
In the FISH assay targeting loci RREB1 (6p25), MYB (6q23), CCND1 (11q13), and CEP (centromere of chromosome 6), 15 of 16 positive cases demonstrated aberrations in chromosome 6 and one case showed gains in 11q13 (CCND1) in their control group of 17 lentiginous nevi, FISH evaluation was negative.
The assay Vysis LSI RREB1/LSI MYB/LSI CCND1/CEP 6 probes, is designed to detect copy number of RREB1 (6p25; red spectrum), MYB (6q23; gold spectrum) and CCND1 (11q13; green spectrum) genes and of centromere 6 (cep6; aqua spectrum) by using FISH in formalin-fixed, paraffin-embedded skin tissue sections (Figure 1).