RPL30

RPL30

A gene on chromosome 8q22 that encodes a protein of the L30E family of ribosomal protein, which is part of the 60S subunit of ribosomes and is located in the cytoplasm. RPL30 is co-transcribed with the U72 small nucleolar RNA gene.
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In the pathogenesis of NAFLD&NASH in Figure 5, we identified eight genes having expression difference between normal liver cells and NAFLD&NASH, i.e., HIST2H2BE (p value [less than or equal to] 1.06 x [10.sup.-1]), RFC5 (p value [less than or equal to] 1.5 x [10.sup.-2]), HSPB1 (p value [less than or equal to] 6.6 x [10.sup.-2]), ZNF480 (p value [less than or equal to] 1.0 x [10.sup.-2]), TUBA1C (p value [less than or equal to] 3.48 x[ 10.sup.-]1), RPL30 (p value [less than or equal to] 1 x [10.sup.-3]), FRAT2 (p value [less than or equal to] 1.08 x [10.sup.-1]), and TRMT1 (p value [less than or equal to] 5.33 x [10.sup.-1]).
In the hepatocarcinogenesis of NAFLD&NASH in Figure 2, we also identified eight genes with differences in expression between NAFLD&NASH and HCC, i.e., HIST2H2BE (p value [less than or equal to] 1.00 x [10.sup.-3]), RFC5 (p value < 1.00 x [10.sup.-3]), HSPB1 (p value [less than or equal to] 1.00 x [10.sup.-3]), ZNF480 (p value [less than or equal to] 1.00 x [10.sup.-3]), TUBA1C (p value [less than or equal to] 1.00 x [10.sup.-3]), RPL30 (p value [less than or equal to] 1.00 x [10.sup.-3]), FRAT2 (p value [less than or equal to] 1.00 x [10.sup.-3]), and ALDOB (p value [less than or equal to] 1.5 x [10.sup.-2]).
Moreover, EGFR also represses TP53 to modulate ETS1 in the MAPK signaling pathway to promote dysfunction of metabolism and apoptosis through the mediation of FRAT2 and RPL30. In addition, the dysregulation of TUBA1C caused by abnormal miR-122 silencing and the dysregulation of ALDOB caused by both hypermethylation and abnormal miR-21 silencing might facilitate tumor metastasis.
In addition, we also found nine genes that play a central role in the upper progression path of Figure 1(a), i.e., two DNA repair-related genes (histone cluster 2 (H2be; HIST2H2BE) and replication factor C5 (RFC5)), four apoptosis-related genes (heat shock 27-kDa protein 1 (HSPB1), zinc finger protein 480 (ZNF480), tubulin alpha 1c (TUBA1C), and ribosomal protein L30 (RPL30)), and three metabolism-related genes (aldolase B (ALDOB), frequently rearranged in advanced T-cell lymphomas 2 (FRAT2), and tRNA methyltransferase 1 (TRMT1)).
In the hepatocarcinogenesis of PBC&PSC in Figure 4, we also identified ten genes having differences in expression between PBC&PSC and HCC, i.e., HIST2H2BE (p value [less than or equal to] 1.00 x [10.sup.-3]), RFC5 (p value [less than or equal to] 1.00 x [10.sup.-3]), TIMP1 (p value [less than or equal to] 1.00 x [10.sup.-3]), ZNF480 (p value [less than or equal to] 1.00 x [10.sup.-3]), H3F3A (p value [less than or equal to] 1.00 x [10.sup.-3]), RPL30 (p value [less than or equal to] 1.00 x [10.sup.-3]), FRAT2 (p value [less than or equal to] 1.00 x [10.sup.-3]), ALDOB (p value < 7.2 x [10.sup.-2]), IGF2 (p value < 1.07 x [10.sup.-1]), and RPL23A (p value [less than or equal to] 1.00 x [10.sup.-3]).
Furthermore, we found seven genes in NAFLD&NASH pathogenesis and NAFLD&NASH-associated hepatocarcinogenesis that also played a central role in PBC&PSC pathogenesis and PBC&PSC-associated hepatocarcinogenesis, i.e., two DNA repair-related genes (HIST2H2BE and RFC5), two apoptosis-related genes (ZNF480 and RPL30), and three metabolism-related genes (ALDOB, FRAT2, and TRMT1).
Furthermore, our finding also demonstrated that epigenetic regulations, including DNA methylation of HSPB1, ALDOB, and RPL30, led to changes in the DNA methylation profiles of HSPB1 (p value [less than or equal to] 1.00 x [10.sup.-3]), ALDOB (p value <[less than or equal to] 0.85), and RPL30 (p value [less than or equal to] 1.00 x [10.sup.-3]), which resulted in significant changes in the gene expression profiles of HSPB1 (p value [less than or equal to] 1.00 x [10.sup.-3]), ALDOB (p value < 1.5 x [10.sup.-2]), and RPL30 (p value < 1.00 x [10.sup.-3]) in the progression from NAFLD&NASH to HCC, as shown in Figure 6.
Our findings demonstrated that epigenetic regulations, including DNA methylation of RPL30, ALDOB, IGF2, and TIMP1, led to changes in the DNA methylation profiles of RPL30 (p value [less than or equal to] 1.00 x [10.sup.-3]), ALDOB (p value [less than or equal to] 0.83), IGF2 (p value [less than or equal to] 1.00 x [10.sup.-3]), and TIMP1 (p value < 1.00 x [10.sup.-3]), which contributed to the significant changes in the gene expression profiles of RPL30 (p value [less than or equal to] 1.00 x [10.sup.-3]), ALDOB (p value [less than or equal to] 7.2 x [10.sup.-2]), IGF2 (p value < 1.1 x [10.sup.-2]), and TIMP1 (p value [less than or equal to] 1.00 x [10.sup.-3]) to progress from PBC&PSC to HCC, as shown in Figure 7.
Lu et al., "Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8," BMC Cancer, vol.
Following NRF2 ChIP, qPCR was done to measure the presence of SPP1 ARE, NQO1 ARE (positive control), and RPL30 Exon 3 (negative control).
That [beta]-actin was in this case valid to use as a housekeeping gene was demonstrated by normalization of proliferating versus differentiating NPCs to three additional housekeeping genes [RPL27, RPL30, OAZ1; see Supplemental Material, Figure 2 (doi: 10.1289/ehp.0901545)].
In the present work, up-regulation of transcripts for several ribosomal proteins such as RPL13a, RPL19, RPL30, RPLP1, RPS24, and RPS26 has been observed.