RNase T1

RNase T1

a nuclease that endonucleolytically cleaves ribonucleic acids at the 3'-5' link of a guanosine 3'-phosphate residue, producing oligonucleotides terminating in this nucleotide; a transferase (endonuclease) in the first (cyclizing) step, a phosphodiesterase on the second (hydrolyzing) step.
Synonym(s): guanyloribonuclease, RNase N1
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References in periodicals archive ?
When RNase T1 was included (Figure 1(b)), transcription was carried out at an NaCl concentration of 150 mM for 20 min.
Inhibition of R-Loop Formation by RNase T1. In order to test whether the transcript was separated from and then reassociated with the template or the RNA stayed on the template, we included a ribonuclease (RNase) in the transcription mixture.
This was resuspended in TE buffer, digested (37 [degrees] C, 30 min) with RNase A and dialyzed RNase T1 (Sigma-Aldrich), followed by addition of 10% sodium dodecyl sulfate and proteinase K for 30 min more.