Plackett-Burman Design (PBD) for screening important medium factors for RNase
The results of this investigation demonstrate the use of a multiplex qPCR assay, the Multiplex TREC qPCR, with an internal QC, the RNase
P gene RPPH1, for use in population-based NBS for detection of SCID.
Of eight families the geneticists suspected of having a prostate cancer-causing gene on chromosome 1, only two have so far revealed mutations in the gene for RNase
L, Carpten and his colleagues report.
The next step in testing this protocol would be a direct comparison between this slot-blot/RPA protocol and a slot blot without RNase
pretreatment using mRNA containing a dilution series of target mRNA.
10/078,949) covers methods of activating a double-stranded RNase
using a double-stranded chemically modified RNA-containing compound with the following properties:
There was complete disappearance of bands from the gel when the RNA sample was treated with RNase
suggestive of the signal in lane 3 to be of RNA (Figure 1, lane 2).
Acknowledgments: We thank Raghavan Varadharajan and Deepak for biotinylation of RNase
A and N.
After centrifugation at 1,600 g for 5 min, cell lysates were incubated with 1% SDS and 5 mg/mL RNase
for 2 hr at 56 [degrees] C, and then with 2.
In addition, Agilent will discontinue sales of its RNase
H minus RT products and Invitrogen will obtain a license from Agilent and pay an undisclosed royalty to sell its DNA polymerase blend products.
To establish that reverse transcription-PCR (RT-PCR) signals were from RNA and not from contaminating DNA, we treated isolated samples with 1 U RNase
ONE Ribonuclease (Promega) per 0.
5 [micro]L 40x MultiScribe (Applied Biosystems) and RNase
inhibitor, each primer (at final concentration of 0.
In October 2001, Invitrogen filed suit against Incyte Genomics alleging infringement of three patents relating to the use of reverse transcriptase with no RNase
H activity for preparing complimentary DNA from RNA.