RNA transcript

RNA transcript

An RNA complementary copy of a gene. The RNA transcript is always longer than the gene because the RNA polymerase also transcribes a leader segment prior to the gene code and a trailer segment after it.
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The technique was developed to accurately quantify DNA or RNA transcript copy number as accurately as possible.
To a first approximation, any PCR-based assay intended for use on a specific DNA target can be modified to work on its RNA transcript by addition of a single preliminary stage to the existing PCR protocol.
The ALK RGQ RT-PCR Kit uses Scorpions technology and contains 2 assays in a single multiplex reaction that enables the detection of RNA transcripts encoding the ALK-TK domain and the control region of the Abelson murine leukemia viral oncogene homolog 1 (ABL1) RNA transcript (ALK RGQ RT-PCR Kit Handbook, Qiagen).
It also became clear that eukaryotic genomes were far more complex than bacterial genomes, both in control structure and in the processing of transcripts (due to the splicing out of introns and the fusing of exons--thus the entire RNA transcript of a locus was not translated).
During gene expression, DNA is first converted into RNA transcript and then some parts of it are removed while others are joined before the trimmed RNA transcript can be translated into proteins.
For example, two adjacent genes can be transcribed together in a single "chimeric" RNA transcript.
Exon: The sequences of the primary RNA transcript (or the DNA that encodes them) that exit the nucleus as part of a messenger RNA (mRNA) molecule.
The primary chimeric RNA transcript in positive sense polarity is produced by the transformed cells from the said promoter.
Commoner buttresses his argument with a lengthy discussion of alternative splicing, but this is a classic red herring: First, any competent biologist knows that the alternative products from any RNA transcript are all directly encoded by the genome.
To test this protocol, three experiments of three runs each were performed using dilution series of 6 to 200 pg of interleukin-2 sense RNA transcript.
RNA interference (RNAi): A RNA transcript produced in the body that does not encode a protein but which can alter the expression of other protein-coding mRNA transcripts; also refers to the process of quelling gene expression through the use of these RNA molecules.