RNA transcript

RNA transcript

An RNA complementary copy of a gene. The RNA transcript is always longer than the gene because the RNA polymerase also transcribes a leader segment prior to the gene code and a trailer segment after it.
References in periodicals archive ?
If an RNA transcript unique to a particular organ is selected, the RNA approach maybe more generally applicable to diseases of that organ and not be limited to the fraction of patients harboring specific DNA signatures.
Traditionally, scientists determine what protein a gene encodes by searching for the often-lengthy RNA transcript, which translates the gene's information into its product.
Plant Bioscience Limited (Norwich, United Kingdom) has patented DNA constructs comprising a promoter operably linked to DNA which can be transcribed in a plant cell to an RNA transcript, wherein the RNA transcript comprises plant virus sequence from an RNA virus which confers on the RNA transcript the ability to replicate in the cytoplasm of the plant cell, wherein the transcript lacks all or part of the viral genome not required for replication in the cytoplasm, and further comprises at least one targeting sequence which is foreign to the plant virus sequence and causes post-transcriptional gene silencing of one or more target genes.
This enzyme recognizes the double-stranded T7 promoter region of the DNA molecule in (e), and drives the production of large numbers of RNA transcript copies of the region.
A quantified CHIKV in vitro RNA transcript containing 9 x [10.sup.10] subgenomic RNA copies/[micro]L was used as a noninfectious positive control.
Such vector may be used to produce an RNA transcript which may be used to immunize a host, including a human host, to protect the host against disease caused by paramyxovirus, particularly respiratory syncytial virus, by administration to the host.
The first step occurs within the nucleus and involves synthesis of the primary RNA transcript or heterogeneous nuclear RNA (hnRNA) [ILLUSTRATION FOR FIGURE 3 OMITTED].
Steps Number of CV (a) replicates RT-gPCR 10 0.12 Isolation + RT-qPCR 8 0.16 Isolation + RT-qPCR 8 0.15 Isolation + RT-qPCR 8 0.18 Isolation + RT-qPCR 8 0.16 Steps Detected RNA, Experimental setup copies RT-gPCR [RNA.sub.LUC] Detection of in vitro RNA transcript Isolation + RT-qPCR [RNA.sub.Luc] RNALuc added to 1 urine sample Isolation + RT-qPCR Endogenous GAPDH Aliquots of 1 urine sample Isolation + RT-qPCR Endogenous RPLPO Aliquots of 1 urine sample Isolation + RT-qPCR Endogenous UBC Aliquots of 1 urine sample (a) Coefficient of intraassay variation.
In brief, the analysis was based on the detection by mass spectrometry of an SNP within an RNA transcript after a primer extension reaction.
PCR-based amplification [switching mechanism at the 5' end of the RNA transcript (SMART)] was originally developed to amplify full-length cDNAs for construction of clone libraries (8, 9), and more recently has been used in microarray experiments (10-12).
In addition, we tested their ability to isolate short RNA fragments in the form of an exogenous, 81-nucleotide, in vitro RNA transcript of the bacterial [beta]-galactosidase ([beta]-gal) gene.
In the MagNA Pure LC protocol for the AMPLICOR HCV Test, ~35% more HCV IC (by volume) was added to the MagNA Pure LC lysis buffer than to the lysis buffer used for the manual method to compensate for the less efficient recovery of this small RNA transcript with the automated method (data not shown).