Ranking UniProtKB Protein name Site Score 1 Q08999 RBL2
S1035 0.4707 2 P28749 RBL1 S964 0.4672 3 P28749 RBL1 T369 0.4481 4 Q08999 RBL2
S672 0.4403 5 P28749 RBL1 S975 0.4389 6 Q08999 RBL2
T401 0.4313 7 Q9UQ35 SRRM2 T1413 0.3952 8 P49736 MCM2 S31 0.3736 9 Q9Y5N6 ORC6 T195 0.3717 10 P24928 POLR2A S1878 0.3663 11 Q15910 EZH2 T487 0.3653 12 Q9UQ35 SRRM2 T866 0.3553 13 O15446 CD3EAP S285 0.3505 14 P24928 POLR2A S1920 0.3495 15 P24928 POLR2A S1934 0.3492 16 Q02539 HIST1H1A S183 0.3488 17 P10276 RARA S77 0.3425 18 Q5TKA1 LIN9 T96 0.3412 19 Q9P1Z0 ZBTB4 T983 0.3347 20 P49736 MCM2 T59 0.3338 Figure 1: Construction of phosphorylation site-kinase network and extracting the pSKN profiles.
IHC analysis of pocket proteins showed that 9-94% of the cells expressed the proliferation associated RB1 and 0-93% expressed the rest of phase protein RBL2. It is thus obvious that there are overlaps in expression of the RB1 and RBL2 proteins.
The Ki67 and cytometry analysis was complemented by an IHC based investigation of the pocket proteins RB1 and RBL2 (also known as P105 and P130).
Compared to the staining intensities for RBL2 and HP1[gamma], the signal was always found weaker in MLS tumor tissues and RB1 staining was also weaker in MLS tissues compared to reference tumor tissues of other entities.
(104-107) Recent genetic-expression profiling studies suggest that silencing of RBL2
, a tumor suppressor gene, in conjunction with MYC overexpression, may be important in the development of BL.
Recently, some miRNAs have been reported to regulate the limitless replicative potential of cancer cells such as the Dicer-dependent miR-290 cluster which can directly target the Retinoblastoma-like 2 protein (Rbl2
) and indirectly affects telomere integrity and telomere-length homeostasis (43).
(b) Gene symbol (c) Gene name (c) 2 PPM [O.sub.3] J02999 Rab2 ras-related protein RAB2 L19698 Rala GTP binding protein (Ral A) X07287 Pkrcg protein kinase C-[gamma] J03552 Mug1 plasma proteinase inhibitor D85760 Gna12 guanine nucleotide-binding protein a-12 M99567 PIcb3 phospholipase C [beta]-3 U00620 Cfs2 GM-CSF M59980 Kcnd2 voltage-gated K+ channel protein M83666 Hck Hck tyrosine protein kinase, p56 AF020777 Ptk2 focal adhesion kinase AF000300 Lyn lyn A tyrosine kinase 5 PPM [O.sub.3] U46034 Mmp11 matrix metal loproteinase 11 D55627 Rbl2
retinoblastoma-like 2 M95738 Slc6a11 Na+/K+ dependent GABA transporter M28647 Atp1a1 Na+/K+ATPaseed subunit U93306 Kdr VEGFR-2 M20637 Plcd1 phospholipase C delta 1 Accession no.