Plant materials: For genomic DNA isolation and subsequent RAPD
analysis, stem of 15 dragon fruit plants were collected from BAU-GPC (Longitude: 24.7175Adeg N, latitude: 90.4310Adeg E).
Genetic diversity, Himalayan gray langur, RAPD
All bulks were subjected to genetic analysis using RAPD
and ISSR techniques to search for differentiating polymorphisms.
To use them, you take extracted DNA from your sample of interest, and set up a "normal" PCR reaction; but in the simplest form of RAPD
, you only put in one PCR primer, and it's one of your randomly chosen 10mers.
Identification of Male Specific Band from RAPD
amplicons showing monomorphic and polymorphic pattern were scored and amplicon size was determined by comparison with 1 kb DNA ladder (Fermentas, U.K.).
Keywords: Agro-morphological traits; Chickpea; Genetic variability; DNA polymorphism; RAPD
Random Amplified Polymorphic DNA (RAPD
) molecular marker systems used in this study are significantly quicker and simpler in comparison to few other molecular strategies, making the technique famous for evaluating genetic polymorphism in wheat species [11,25].
Also, 15 RAPD
primers were applied; only 10 of them (Table 3) were amplified and showed polymorphism.
Also, the PCR product using selected RAPD-PCR marker assisted analysis gave an evidence of DNA damage and mutations which was reflected in their RAPD
profiles such as disappearance of bands and appearance of new PCR products which occurred in the profiles generated by rats exposed to Wi-Fi radiation.
analysis: Eighteen decamer random primers of OPA, OPB and OPP series (Operon Technologies, USA: http://www.operon.com) were used for initial screening of all the isolates.