RAMP1


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RAMP1

A gene on chromosome 2q36-q37.1 that encodes one of the RAMP (receptor [calcitonin] activity-modifying proteins) family of single-transmembrane-domain proteins, which are type-I transmembrane proteins needed to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on the expressed RAMP; in the presence of RAMP1, CRLR acts as a CGRP receptor. RAMP1 protein is involved in the terminal glycosylation, maturation and presentation of the CGRP receptor to the cell surface.
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For quantitative analysis of the genes of interest [pre-pro-AM (Rn 00562327_m1), CRLR (Rn 00562334_m1), RAMP1 (Rn 01427056_m1), RAMP2 (Rn 00824652_m1), and RAMP3 (Rn 00571815_m1)], a commercially available TaqMan Assay-on-Demand System that consists of a kit of oligonucleotides and probes was used (Applied Biosystems).
The membranes were then incubated with the following specific antibodies (Santa Cruz Biotechnology, USA) overnight at 4[degrees]C: AM (sc-16496, 1:250 dilution), CRLR (sc-18007, 1:250), RAMP1 (sc-11379, 1:250), RAMP2 (sc-11380, 1:250), and RAMP3 (sc-11381, 1:250).
Figure 1A shows representative immunoblots for AM, CRLR, and RAMP1, -2, and -3 protein expression in rat CSM.