This enzyme has been isolated from many organisms, but only the one from Pseudomonas diminuta was cloned and expressed in a recombinant E.
found a mutant of CPC acylase activity from Pseudomonas diminuta N176, Ala675Gly showed 1.12-fold compared with its wild type (7); Golden et al.
by southern hybridization with opd from Pseudomonas diminuta
Raushel, "Structure-activity relationships in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta," Biochemistry, vol.
Raushel, "Purification and properties of the phosphotriesterase from Pseudomonas diminuta," The Journal of Biological Chemistry, vol.
Raushel, "Detoxification of organophosphate pesticides using a nylon based immobilized phosphotriesterase from Pseudomonas diminuta
," Applied Biochemistry and Biotechnology, vol.
Gibson (1985).Enzymatic hydrolysis of organophosphates: cloning and expression of a parathion hydrolase gene from Pseudomonas diminuta
I 1/1 1/1 Pseudomonadaceae I,P 1/11 1/11 Pseudomonas diminuta
I 0/1 0/1 P.
We ourselves reported a squid-like enzyme from Pseudomonas diminuta
that hydrolyzes the P-S bond of the VX-type nerve agents ([R.sub.1][R.sub.2]P(= O)S[R.sub.3]) and, more to the point, hydrolyzes DFP about eight times faster than Soman (2).
FDA defines a sterile filter as "A filter when challenged with bacterial pseudomonas diminuta
at a concentration of atleast 1x10 to the power 7 per square centimeter of the effective filter area produces a sterile effluent."
Recently we reported an organophosphorus hydrolase (OPH) from Pseudomonas diminuta
that hydrolyzes VX and Tetriso, and also DFP and Soman (2).
The purification of an enzyme, organophosphorus hydrolase (OPH), derived from Pseudomonas diminuta
through genetic manipulations and biochemical alterations, is the work of Wild and colleagues (5) who have also made the enzyme available to us.