chromosome walking(redirected from Primer walking)
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Related to Primer walking: Chromosome walking
sequential isolation of overlapping sequences of DNA (that is, clones); with this procedure large regions of the chromosome can be spanned.
Synonym(s): overlap hybridization
Farlex Partner Medical Dictionary © Farlex 2012
chromosome walkingA procedure for determining the nucleotide sequence of segments of DNA that are located on either side of a region of fully characterised (i.e., “sequenced”) DNA. Chromosome walking requires the dividing (“walking out”) of each segment of DNA into overlapping short (15–20,000) bases from a point of a known sequence; this enables one to sequence DNA far removed from a marker gene.
Segen's Medical Dictionary. © 2012 Farlex, Inc. All rights reserved.
chro·mo·some walk·ing(krō'mŏ-sōm wawk'ing)
A process of extending a genetic map by successive hybridization steps.
Medical Dictionary for the Health Professions and Nursing © Farlex 2012
chromosome walkinga technique for mapping chromosomes that involves the sequential isolation of clones carrying overlapping NUCLEOTIDE SEQUENCES. This makes it possible to ‘walk’ along the chromosome from one gene to the next, to reach a desired LOCUS. Chromosome walking is used in genome sequencing projects, see CLONE CONTIG SEQUENCING METHOD.
Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005