Populations of Fibrobacter succinogenes, Ruminococcus albus, Streptococcus bovis, Prevotella ruminicola
, Prevotella albensis, Eubacterium ruminantium, Anaerovibrio lipolytica, Ruminococcus flavefaciens, methanogenic archaea, general protozoa, and general fungi were analyzed using a previously described quantitative PCR method [12-16].
Morrison et al., "Comparative genome analysis of Prevotella ruminicola
and Prevotella bryantii: insights into their environmental niche," Microbial Ecology, vol.
Flint, "A xylan hydrolase gene cluster in prevotella ruminicola
B14: sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and [beta]-(1,4)-xylosidase activities," Applied and Environmental Microbiology, vol.
The high percentage of Prevotella, mainly the species Prevotella ruminicola
, Prevotella brevis, Prevotella bryantii and Prevotella albensis (McSweeney & Mackie, 2012), revealed the genus's abundance in the rumen (Bekele et al., 2010; Stevenson & Weimer, 2007).
The glucose toxicity of Prevotella ruminicola
: methylglyoxal accumulation and its effect on membrane physiology.
abundance tended to increase in a linear manner by EOM supplementation (p = 0.080).
In contrast, Prevotella ruminicola
, Butyrivibrio proteoclasticus were the two most abundant species associated with cellulases activity in the Korean goat rumen study .
Fibrobacter succinogenes, Butyrivibrio fibrisolvens, Ruminococcus albus, Ruminococcus flavefaciens, Prevotella ruminicola
, and Actinomyces are the small group of microorganisms used for the gene cloning .
Tajima et al  designed 12 primer sets specific for culturable ruminal bacterial species that include Fibrobacter succinogenes, Ruminococcus flavefaciens, Ruminococcus albus, Prevotella ruminicola
, Prevotella albensis, Prevotella bryantii, Selenomonas ruminantium-Mitsuokella multiacida, Streptococcus bovis, Eubacterium ruminantium, Treponema bryantii, Succinivibrio dextrinosolvens, and Anaerovibrio lipolytica.
Primer used for the real-time polymerase chain reaction Gene name Primer sequence 5'-3' Amplicon size (bp) General bacteria F:CGGCAACGAGCGCAACCC 130  R:CCATTGTAGCACGTGTGTAGCC Ruminococcus F:CGAACGGAGATAATTTGAGTTTACTTAGG 132  flavefaciens R:CGGTCTCTGTATGTTATGAGGTATTACC Butyrivibrio F:ACCGCATAAGCGCACGGA 65  fibrisolvens R:CGGGTCCATCTTGTACCGATAAAT Prevotella ruminicola
F:GCGAAAGTCGGATTAATGCTCTATG 78  R:CCCATCCTATAGCGGTAAACCTTTG Lactobacillus spp.