Ingenuity Pathway Analysis showed the following biological functions to be affected in the HPV+ samples: cancer, hematological disease, and RNA post-transcriptional modification
. QRT-PCR analysis confirmed only the differential regulation of ALYREF, KEAP1, and FAM186B genes.
Of all the RNAs, tRNA exhibits the largest number and most diverse modifications with cells from all kingdoms of life allocating a large portion of their genome to encoding enzymes involved in the post-transcriptional modification
of nucleosides in tRNA .
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are complexes of RNA and protein present in the nucleus during gene transcription and subsequent post-transcriptional modification
of the newly synthesised RNA (pre-mRNA).
(7) An alternative explanation for the low CK levels is that a generalised inflammatory state may result in post-transcriptional modification
and enhanced degradation of CK.
This project will focus on investigating the haustorial and the EHM proteomes of first Blumeria / barley, then followed by other biotrophic interactions, with the additional aim to reveal early events of biotic stress perception and signalling that transcriptome analysis cannot, by studying post-transcriptional modifications
, protein interactions and relocation to different cell compartments like haustoria or lipid rafts.
of proteins occur by all of the following EXCEPT
are commonly found in non-coding RNA species, yet the biological function is unknown.