phosphotungstic acid

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phos·pho·tung·stic ac·id (PTA),

(fos-fō-tŭng'stik as'id),
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phos·pho·tung·stic ac·id

(PTA) (fos'fō-tŭng'stik as'id)
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phos·pho·tung·stic ac·id

(PTA) (fos'fō-tŭng'stik as'id)
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.
References in periodicals archive ?
The virus preparation was negative-stained with sodium phosphotungstate (original magnification *25,000).
The blue coloured complex developed due to the reduction of phosphomolybdate and phosphotungstate by tyrosine residues of polypeptide was estimated spectrophotometrically at 680 nm.
Li et al., "Microwave radiation solidphase synthesis of phosphotungstate nanoparticle catalysts and photocatalytic degradation of formaldehyde," Journal of Molecular Catalysis A, vol.
Estimation of HDL-C was done using magnesium chloride and sodium phosphotungstate to precipitate low density and very low-density lipoproteins, which were then removed by centrifugation at 3000rpm.
The precipitation reagent (sodium phosphotungstate with magnesium chloride) was added to the sera to aggregate non-HDL lipoproteins, which were then sedimented by centrifugation (10,000 g for 5 minutes).
Serum HDL was measured after precipitation of LDL and VLDL by use of heavy metals salts like phosphotungstate) Mg++ (using cholesterol kit reagents cod.
For transmission electron microscopy (EM) raw stool samples were processed by super direct negative staining with 2% sodium phosphotungstate, pH 6.3, as described elsewhere.
The following regents were used for the assay: (a) Reagent 1: Sulphuric acid, 2/3 N; (b) Reagent 2: Sodium tungstate, 10% W/V; (c) Reagent 3: Sodium carbonate, 14% W/V; (d) Reagent 4: Phosphotungstate; (e) Reagent 5: Stock Uric acid standard, 100 mg%; Working Standard was prepared with dilution of 0.1 ml of stock Uric acid standard to 10 ml purified water and mixed well.
Serum LDl-[HDL.sub.1] were isolated by precipitation using [MgCl.sub.2] and phosphotungstate (Sigma Chemical Company, France) by the method of Burstein et al.
A uric acid evaluation, for example, would summarize survey results for the uricase, phosphotungstate, and iron reduction methods.
Estimation of cholesterol and HDL cholesterol was done by CHOD--POD phosphotungstate method.

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