phosphotungstic acid

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phos·pho·tung·stic ac·id (PTA),

(fos-fō-tŭng'stik as'id),
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phos·pho·tung·stic ac·id

(PTA) (fos'fō-tŭng'stik as'id)
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phos·pho·tung·stic ac·id

(PTA) (fos'fō-tŭng'stik as'id)
A mixture of phosphoric and tungstic acids; a protein precipitant and reagent for arginine, lysine, histidine, and cystine; used with hematoxylin for nuclear and muscle staining; also used in electron microscopy as a stain for collagen and as a negative stain.

phosphotungstic acid (PTA),

n a mixture of phosphoric and tungstic acids used with hematoxylin for staining muscle tissue and cell nuclei. It is also used as a negative stain of collagen in electron microscopy.
References in periodicals archive ?
We measured HDL cholesterol after precipitation of the apolipoprotein B-containing lipoproteins with magnesium phosphotungstate and calculated LDL cholesterol by the Friedewald formula.
25), using a special phosphotungstate preconcentration step, found [PrP.
Different methods have been used for this, including spectrophotometric measurement of cysteine in urine with phosphotungstate used as a chromogen and by HPLC followed by ultraviolet detection.
The HDL-cholesterol concentration was determined enzymatically by measuring cholesterol in the supernatant after precipitation with phosphotungstate.
HDL-cholesterol was determined after precipitation of the apolipoprotein B-containing lipoproteins with magnesium phosphotungstate.
Specifications in the HDL-C assay kit indicate that reagent 1 includes both a polyanion and a synthetic polymer; phosphotungstate is used as the polyanion.
Thus, we confirmed that phosphotungstate in reagent 1 is contained in aggregates of VLDL.
The poor analytical recovery of the PPD method with the latter materials could be attributable to our use of a different reagent system than the one recommended by the manufacturer (who specifies that only cholesterol-precipitating reagents containing phosphotungstate may be used with Lin-Trol).
Colwell We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and [Mg.

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