with increased expression level was identified as dihydropyrimidinase-related protein 2, and 2 phosphoproteins
with decreased expression level were identified as proteasome subunit alpha type-3 and beta-soluble NSF attachment protein (Table 2).
We employed a combination of biochemical and histological techniques to test the hypothesis that the barnacle adhesive interface contains phosphoproteins
. Specifically, we aimed to identify phosphoproteins
in uncured barnacle glue and to determine their location within the barnacle adhesive interface, using residual glue deposited by reattaching barnacles and decalcified barnacle base plates.
A total of 1,645 phosphoprotein
groups were identified by scaffold software across all six TNBC cell lines, using peptide probability thresholds of 95% with a minimum of one unique peptide (Figure 1(a)).
kinase C and protein F1: Protagonists of plasticity in particular pathways.
Tissue lysates were immunoblotted, and total protein and phosphoprotein
levels of Syk, IKK, I[kappa]B [alpha], p65, and [beta]-actin were determined, as previously reported [44, 45].
Of 55 metabolic phosphoproteins
, the top two pathways are ribosome and focal adhesion pathways [Figure 3].
They selected 88 antibodies that recognize proteins and phosphoproteins
involved in a variety of cancer cell functions such as kinase signaling, epithelial-mesenchyme transition, apoptosis, and autophagy.
Both isozymes are known to be phosphoproteins
A sampling of topics: application of pressure cycling technology (PCT); immunoaffinity depletion of high-abundant proteins; new ultrafiltration and solid phase extraction techniques to improve serum peptide detection; optic nerve fractionation; destreaking strategies for two-dimensional electrophoresis; proteomic approaches to the study of rhematoid arthritis; structural approaches in glycoproteomics; polymeric monolithic capillary columns in proteomics; proteins staining in polyacrylamide gels; and fluorescent detection of proteins, glycoproteins, and phosphoproteins
in two-dimensional gels.
of oyster (Crassostrea virginica) shell organic matrix.
Such associative behavior of other biomineral proteins such as dentin phosphophoryn, as well as other polyanionic proteins such as casein, has been reviewed by Marsh (1989a, b), who also observed agglomerations of phosphoproteins
and mineral salts including amorphous calcium phosphate in nonmineralizing compartments of some molluscs (Marsh and Sass, 1983, 1984, 1985).
Profile and Western Blot in Endothelial Cells.