The development of a new culture medium for intestinal protozoan allows learning about the biology of parasites, their growth rate, virulence factors (like phospholipase
activity), susceptibility to new drugs, and the appearance of resistant strains, among other aspects .
Marcussi, "Inhibition of proteases and phospholipases
A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins," Anais da Academia Brasileira de Ciencias, vol.
In addition, many snake venoms have in common active toxic components that could be targeted by SMTs, including the secreted phospholipase
A2 ([sPLA.sub.2]) and metallo- and serineproteases (svMPs and SPs, respectively).
[A.sub.2] biochemistry," Cardiovascular Drugs and Therapy, vol.
fumigatus to produce extracellular phospholipases
. Aspergillosis is a disease almost exclusively acquired by inhalation of airborne conidia which penetrate deep into the alveolar spaces that are lined with lung surfactant which is composed of up to 80% phospholipid.
(2008) Biochemistry and physiology of mammalian secreted phospholipases
The comparison of 50 amino acids residues from Bmoo-SII placed it in the phospholipase
[A.sub.2] superfamily with high identity to Myotoxin II from B.
albicans, which though distantly related have similar gene families encoding for enzymes phospholipases
, lipases, aspartyl proteases, and acid sphingomyelinases.
The determination of phospholipase
activity was performed using the egg yolk agar plate method (2% glucose, 1% peptone, 0.5% yeast extract, 4% NaCl, 0.074% Ca[Cl.sub.2], 1.5% agar, then, 2% of fresh egg yolk was added to the medium) as previously described by Price et al.
These studies also reported that melittin has significant lipid regulating activities by activating phospholipase
D (PLD, EC 18.104.22.168) is a lipolytic enzyme which has gradually emerged, over recent decades, as one of the key components of a broad range of cellular and physiological processes (see  for review).
The enzymes, such as hyaluronidase, phospholipase
[A.sub.2] ([PLA.sub.2]), [alpha]-D-glucosidase, lysopphospholipase, [alpha]-acetylamino-deosiglucosidase, arylamidase and phosphomonoesterase acid esterase.