The components are (1) tumor markers: HCG-lntact Hormone by IRMA, HCG-Total Hormone (intact, nicked intact, free [beta], nicked, and free [beta], fragments) by chemiluminescence, HCG-Urine test by chemiluminescence, PHI enzyme (phosphohexose isomerase or glucose phosphate isomerase) by enzyme kinetics, and CEA (carcinoembryonic antigen) by chemiluminescence; and (2) peripherally related assays: GGTP, TSH, and DHEA-S.
PHI: Phosphohexose isomerase, EC 22.214.171.124, is also known as glucose phosphate isomerase or phosphoglucose isomerase, and was originally isolated from a human melanoma cell line.
Enzyme activity Ensyme C2-4 (normal) Ex139 (hls) units [g.zup.-1] protein Amylase 34.7 35.3 Starch phosphorylase 15.7 15.3 Hexokinase 0.95 1.01 Phosphoglucomutase 0.84 0.80 Phosphohexose isomerase
1.88 1.87 Phosphofruetokinase 0.84 0.83 Aldolase 6.9 6.5 Triosephosphate isomerase 125 126 Fructose-1,6-bisphosphatase 2.8 2.7 UDP glucose pyrophosphorylase 0.5 0.5 Sucrose-P phosphalase 18 17 Invertase 61.4 87.3 Significance Ensyme of t-test Amylase NS Starch phosphorylase NS Hexokinase NS Phosphoglucomutase NS Phosphohexose isomerase
NS Phosphofruetokinase NS Aldolase NS Triosephosphate isomerase NS Fructose-1,6-bisphosphatase NS UDP glucose pyrophosphorylase NS Sucrose-P phosphalase NS Invertase (**) (*), (**) means significantly different at P = 0.05 and P = 0.01, respectively.