Assays were performed in a triethanolamine--HCl buffer solution (100 mM; pH, 7.6) completed with phosphoglucose isomerase
(1.2 U x [mL.sup.-1]), glucose-6-phosphate dehydrogenase (0.5 U x [mL.sup.-1]), NADP (0.4 mM), and mannose-6-phosphate (3.2 mM).
Abbreviations: ADH, alcohol dehydrogenase; APS, acid phosphatase; EST, esterase; GOT, glutamate oxaloacetate transaminase; MDH, malate dehydrogenase; 6-PGDH, 6-phosphogluconate dehydrogenase; PGI, phosphoglucose isomerase
; PGM, phosphoglucomutase; PRX, anodal peroxidase; SDH, shikimate dehydrogenase; TPI, triophosphate isomerase.
The enzymes phosphoglucose isomerase
(PGI, E.C.188.8.131.52), phospho-glucomutase (PGM, E.C.184.108.40.206), glutamate oxaloacetate transaminase (GOT, E.C.220.127.116.11.), and triosphosphate isomerase (TPI, E.C.18.104.22.168) were resolved by means of stains prepared according to the methods of Vallejos (1983).
Yamamoto and Duich (1994) were able to identify 12 of 12 synthetic creeping bentgrass cultivars based on phosphoglucose isomerase
The adaptive significance of electrophoretic mobility in phosphoglucose isomerase
22.214.171.124), Phosphoglucose isomerase
(PGI; EC no.126.96.36.199), Phosphoglucomutase (PGM; EC no.
I assayed three loci - hexokinase (Hk, EC 188.8.131.52), phosphoglucose isomerase
(Pgi, EC 184.108.40.206), and triosephosphate isomerase (Tpi, EC 220.127.116.11) - using the pH 8.0 Tris-citrate buffer system of Selander et al.
The 13 enzyme systems (and 18 associated, polymorphic loci) assayed for this study were acid phosphatase (Acp), diaphorase (Dia2), fluorescent esterase (Fe2, Fe3), glutamate oxaloacetate transaminase (Got1, Got2), isocitrate dehydrogenase (Idh), leucine aminopeptidase (Lap1, Lap2), malate dehydrogenase (Mdh), malic enzyme (Me), 6-phosphogluconate de hydrogenase (6Pgd1, 6Pgd2), phosphoglucose isomerase
(Pgi2), phosphoglucomutase (Pgm1, Pgm2), shikimate dehydrogenase (Skdh), and triosphosphate isomerase (Tpi1).
1980) was used to resolve phosphoglucose isomerase
(Pgi-2), menadione reductase (Mnr-1), and triosephosphate isomerase (Tpi-2).
These enzymes included alanine transferase (Alt-2); fluorescent esterase (Fle-2); glutamate oxaloacetate transaminase (Got-1, Got-2); glutamate dehydrogenase (Gdh-1); isocitrate dehydrogenase (Idh-1); leucine aminopeptidase (Lap-1); malate dehydrogenase (Mdh-1, Mdh-2); menadione reductase (Mnr-1, Mnr-2); phosphoglucomutase (Pgm-1); 6-phosphogluconate dehydrogenase (Pgd-1); phosphoglucose isomerase
(Pgi-2); and triosephosphate isomerase (Tpi-1, Tpi-2).
Results were obtained from 11 enzyme systems, including aldolase (ALD), fructose-bisphosphatase (FBP), glutamate oxaloacetate transaminase (GOT), hexokinase (HK), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), 6-phosphogluconate dehydrogenase (6PGDH), phosphoglucose isomerase
(PGI), shikimate dehydrogenase (SkDH), and triosephosphate isomerase (TPI).
Of 53 enzyme assays tried, the following gave scorable results (designation of presumptive loci in parentheses): Alkaline phosphatase (Alkph-1 and Alkph-2), Esterases (Est-1 and Est-2), N-Acetyl-[Beta]-glucosaminase ([Beta]Ga), Fructokinase (Fk), Fumarate dehydrogenase (Fum), Glutamate-oxaloacetate transaminase (Got), Glutamic dehydrogenase (Glutdh), [Alpha]-Glycerophosphate dehydrogenase ([Alpha]Glyphdh), Mannose-6-phosphate isomerase (M6pi), NAD+ dependent Malate dehydrogenase (Mdh-1 and Mdh-2), L-Leucyl-L-Tyrosine peptidase (Peplt), Phosphoglucose isomerase
(Pgi), Phosphoglucomutase (Pgm), Triosephosphate isomerase (Tpi).